Overview
- External ontology
- TOGO:M2312 — Castenholz TYE medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2312 Original URL: https://www.atcc.org/~/media/40D6184E15BC4586AE200B0FB00A64CE.ashx
Composition
flowchart LR
CultureMech_008899["castenholz_tye_medium"]
ing0_DI_Water["DI Water"]
CultureMech_008899 --> ing0_DI_Water
ing1_Agar__alternative_["Agar (alternative)"]
CultureMech_008899 --> ing1_Agar__alternative_
ing2_Castenholz_Salts__2x["Castenholz Salts, 2x"]
CultureMech_008899 --> ing2_Castenholz_Salts__2x
ing3_NaCl["NaCl"]
CultureMech_008899 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_NaNO3["NaNO3"]
CultureMech_008899 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008899 --> ing5_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing5_Nitrilotriacetic_acid -.-> CHEBI_44557
ing6_KNO3["KNO3"]
CultureMech_008899 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
ing7_Na2HPO4["Na2HPO4"]
CultureMech_008899 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8_CaSO4___2H2O["CaSO4 · 2H2O"]
CultureMech_008899 --> ing8_CaSO4___2H2O
CHEBI_32583["CHEBI:32583"]
ing8_CaSO4___2H2O -.-> CHEBI_32583
ing9_MgSO4_H2O["MgSO4·H2O"]
CultureMech_008899 --> ing9_MgSO4_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_H2O -.-> CHEBI_31795
ing10_Agar__if_needed_["Agar (if needed)"]
CultureMech_008899 --> ing10_Agar__if_needed_
ing11_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008899 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_008899 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008899 --> ing13_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2_6H2O -.-> CHEBI_35696
ing14_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008899 --> ing14_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_7H2O -.-> CHEBI_32312
ing15_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008899 --> ing15_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_5H2O -.-> CHEBI_31440
ing16_H2SO4["H2SO4"]
CultureMech_008899 --> ing16_H2SO4
CHEBI_26836["CHEBI:26836"]
ing16_H2SO4 -.-> CHEBI_26836
ing17_MnSO4["MnSO4"]
CultureMech_008899 --> ing17_MnSO4
CHEBI_86360["CHEBI:86360"]
ing17_MnSO4 -.-> CHEBI_86360
ing18_FeCl3["FeCl3"]
CultureMech_008899 --> ing18_FeCl3
CHEBI_30808["CHEBI:30808"]
ing18_FeCl3 -.-> CHEBI_30808
ing19_Yeast_extract["Yeast extract"]
CultureMech_008899 --> ing19_Yeast_extract
ing20_Tryptone__BD_211705_["Tryptone (BD 211705)"]
CultureMech_008899 --> ing20_Tryptone__BD_211705_
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | DI Water | 3500.0 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 400.0, 1000.0, 1000.0, 100.0, 1000.0] | |
| 2 | Agar (alternative) | 30 G_PER_L | Role: Solidifying component | |
| 3 | Castenholz Salts, 2x | 500 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.016 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
1.4 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 7 |
KNO3
K.NO3 101.102 g/mol
|
0.21 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.22 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CaSO4 · 2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.12 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MgSO4·H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Agar (if needed) | 30 G_PER_L | Role: Solidifying component | |
| 12 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.046 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.5 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.016 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H2SO4
H2O4S 98.079 g/mol
|
0.5 G_PER_L |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
| 18 |
MnSO4
Mn.O4S 151.001 g/mol
|
2.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.03 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 | Yeast extract | 10 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 21 | Tryptone (BD 211705) | 10 G_PER_L | Properties: Undefined component |
Solutions
1% TYE Solution
Dose: 100 G_PER_L
Nitsch's Trace Elements (see below)
Dose: 2 G_PER_L
FeCl3 solution (0.03%, see below)
Dose: 2 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M2312_Castenholz_TYE_medium (CultureMech:008899)
- castenholz_tye_medium (CultureMech:008898)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.323450Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2312 |
| 2026-01-28T19:48:35.816048Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:59.640748+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:31.396126Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:01.147793Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008899 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:19.852391+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Castenholz TYE medium" to "castenholz_tye_medium" for consistent matching |
| 2026-05-16T09:03:12.992877+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.187583+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.836437+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.151036+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M2312_Castenholz_TYE_medium, castenholz_tye_medium |
| 2026-08-06T03:05:11.151075+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 1170edcbc387de536de4f941edb9e4abcdd5725a0df58c7c35930200d0dafaf3 |