Overview
- External ontology
- TOGO:M2334 — Chthonomonas Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2334 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1291.pdf
Composition
flowchart LR
CultureMech_008921["chthonomonas_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008921 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008921 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_008921 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_008921 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_008921 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Gellan_Gum__Phytagel_["Gellan Gum (Phytagel)"]
CultureMech_008921 --> ing5_Gellan_Gum__Phytagel_
ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008921 --> ing6_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_008921 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_008921 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_008921 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_008921 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_008921 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_ZnCl2["ZnCl2"]
CultureMech_008921 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_008921 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_008921 --> ing14_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing14_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing15_NaOH["NaOH"]
CultureMech_008921 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008921 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_008921 --> ing17_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_pantothenate -.-> CHEBI_31345
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_008921 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_008921 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_008921 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_008921 --> ing21_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing22_D____Biotin["D(+)-Biotin"]
CultureMech_008921 --> ing22_D____Biotin
CHEBI_15956["CHEBI:15956"]
ing22_D____Biotin -.-> CHEBI_15956
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 1000.0, 1000.0] |
| 2 | Yeast extract | 0.01 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Gellan Gum (Phytagel) | 0.5 G_PER_L | Role: Solidifying component; Properties: Organic compound, Undefined component | |
| 7 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
190 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
Properties: Defined component, Organic compound, Simple component |
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
80 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
100 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
300 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 | Vitamin B12 | 100 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
200 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
200 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
CaCl2 x 2 H2O solution
Dose: 0.15 G_PER_L
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution
Trace element solution SL-11
Dose: 1 G_PER_L
See mediadive_5191_Trace_element_solution_SL-11.yaml for composition
Seven vitamins solution
Dose: 1 G_PER_L
See mediadive_4611_Seven_vitamins_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2334_Chthonomonas_Medium (CultureMech:008921)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.357512Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2334 |
| 2026-01-28T19:48:34.230460Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:56.796940+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:28.375472Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:01.294222Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008921 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.009095+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chthonomonas Medium" to "chthonomonas_medium" for consistent matching |
| 2026-05-16T09:03:13.081502+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.288169+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.860890+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.245113+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d7de88257d78f6fc94acbedc40ff7712f3b4cedc2f10aadf7da479b951e26b14 |