Overview
- External ontology
- TOGO:M236 — Stetteria Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M236 Original source: JCM - JCM_M244 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=244
Composition
flowchart LR
CultureMech_008954["stetteria_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008954 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_008954 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Resazurin["Resazurin"]
CultureMech_008954 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2S_9H2O["Na2S・9H2O"]
CultureMech_008954 --> ing3_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_9H2O -.-> CHEBI_76209
ing4_NaHCO3["NaHCO3"]
CultureMech_008954 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008954 --> ing5_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing5_Sulfur__powder_ -.-> CHEBI_33403
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008954 --> ing6_Yeast_extract__BD_Difco_
ing7_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_008954 --> ing7_Bacto_peptone__BD_Difco_
ing8_Synthetic_seawater__2_x__see_below_["Synthetic seawater (2 x, see below)"]
CultureMech_008954 --> ing8_Synthetic_seawater__2_x__see_below_
ing9_H2SO4["H2SO4"]
CultureMech_008954 --> ing9_H2SO4
CHEBI_26836["CHEBI:26836"]
ing9_H2SO4 -.-> CHEBI_26836
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008954 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_N2["N2"]
CultureMech_008954 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
ing12_Hydrogen_gas["Hydrogen gas"]
CultureMech_008954 --> ing12_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing12_Hydrogen_gas -.-> CHEBI_18276
ing13_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008954 --> ing13_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_7H2O -.-> CHEBI_31795
ing14_NaCl["NaCl"]
CultureMech_008954 --> ing14_NaCl
CHEBI_26710["CHEBI:26710"]
ing14_NaCl -.-> CHEBI_26710
ing15_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008954 --> ing15_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_2H2O -.-> CHEBI_86158
ing16_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008954 --> ing16_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing16_MgCl2_6H2O -.-> CHEBI_86345
ing17_H3BO3["H3BO3"]
CultureMech_008954 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_KCl["KCl"]
CultureMech_008954 --> ing18_KCl
CHEBI_32588["CHEBI:32588"]
ing18_KCl -.-> CHEBI_32588
ing19_NaBr["NaBr"]
CultureMech_008954 --> ing19_NaBr
CHEBI_63004["CHEBI:63004"]
ing19_NaBr -.-> CHEBI_63004
ing20_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_008954 --> ing20_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing20_SrCl2_6H2O -.-> CHEBI_36385
ing21_KI["KI"]
CultureMech_008954 --> ing21_KI
CHEBI_8346["CHEBI:8346"]
ing21_KI -.-> CHEBI_8346
ing22_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_008954 --> ing22_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_6H2O -.-> CHEBI_34887
ing23_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_008954 --> ing23_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_2H2O -.-> CHEBI_63939
ing24_Na2SeO3["Na2SeO3"]
CultureMech_008954 --> ing24_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing24_Na2SeO3 -.-> CHEBI_48843
ing25__NH4_2Ni_SO4_2_6H2O["(NH4)2Ni(SO4)2・6H2O"]
CultureMech_008954 --> ing25__NH4_2Ni_SO4_2_6H2O
CHEBI_86149["CHEBI:86149"]
ing25__NH4_2Ni_SO4_2_6H2O -.-> CHEBI_86149
ing26_Na2SeO4["Na2SeO4"]
CultureMech_008954 --> ing26_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing26_Na2SeO4 -.-> CHEBI_77775
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
502.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 500.0, 1.0, 1.0] |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.75 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 4 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 8 | Bacto peptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 | Synthetic seawater (2 x, see below) | 500 G_PER_L | ||
| 10 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 14 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
14 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaCl
Cl.Na 58.443 g/mol
|
55.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.06 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
KI
I.K 166.002 g/mol
|
0.1 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
25 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
10 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.3 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
(NH4)2Ni(SO4)2・6H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
10 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace minerals (see Medium [M142])
Dose: 15 G_PER_L
Cross-reference to Medium M142
Ni-Se-W solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M236_Stetteria_Medium (CultureMech:008954)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.678220Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M236 |
| 2026-01-28T19:48:26.357065Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:43.755431+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:13.921699Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:01.550951Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008954 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.275056+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Stetteria Medium" to "stetteria_medium" for consistent matching |
| 2026-05-16T09:03:13.304298+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.541163+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.236793+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:26.393072+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.943325+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.335812+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0a06dac33f968b224c81ecdaaffa34ecbc7498257e2aeacc3321dcfccaabcb32 |