Overview
- External ontology
- TOGO:M2378 — Natronobacteria medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2378 Original URL: https://www.atcc.org/~/media/8386F32049764A12AC1B1893925E9DF9.ashx
Composition
flowchart LR
CultureMech_008962["natronobacteria_medium"]
ing0_Distilled_water_to["Distilled water to"]
CultureMech_008962 --> ing0_Distilled_water_to
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_008962 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008962 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008962 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KH2PO4["KH2PO4"]
CultureMech_008962 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_008962 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_KCl["KCl"]
CultureMech_008962 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_CaSO4___2_H2O["CaSO4 · 2 H2O"]
CultureMech_008962 --> ing7_CaSO4___2_H2O
CHEBI_32583["CHEBI:32583"]
ing7_CaSO4___2_H2O -.-> CHEBI_32583
ing8_Sodium_glutamate["Sodium glutamate"]
CultureMech_008962 --> ing8_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing8_Sodium_glutamate -.-> CHEBI_64220
ing9_Agar["Agar"]
CultureMech_008962 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Casamino_acids["Casamino acids"]
CultureMech_008962 --> ing10_Casamino_acids
ing11_Distilled_water["Distilled water"]
CultureMech_008962 --> ing11_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing11_Distilled_water -.-> CHEBI_15377
ing12_Na2MoO4_H2O["Na2MoO4.H2O"]
CultureMech_008962 --> ing12_Na2MoO4_H2O
CHEBI_75215["CHEBI:75215"]
ing12_Na2MoO4_H2O -.-> CHEBI_75215
ing13_H3BO3["H3BO3"]
CultureMech_008962 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2___4H2O["MnCl2 · 4H2O"]
CultureMech_008962 --> ing14_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2___4H2O -.-> CHEBI_86368
ing15_CoCl2___6H2O["CoCl2 · 6H2O"]
CultureMech_008962 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_NiCl2___6H2O["NiCl2 · 6H2O"]
CultureMech_008962 --> ing16_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2___6H2O -.-> CHEBI_34887
ing17_ZnSO4___7H2O["ZnSO4 · 7H2O"]
CultureMech_008962 --> ing17_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4___7H2O -.-> CHEBI_32312
ing18_CuCl2___2H2O["CuCl2 · 2H2O"]
CultureMech_008962 --> ing18_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2___2H2O -.-> CHEBI_86318
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Distilled water to | 1 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.24 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaSO4 · 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.17 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 10 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 11 | Casamino acids | 5 G_PER_L | Properties: Undefined component | |
| 12 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2MoO4.H2O
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 · 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2 · 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 · 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4 · 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2 · 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Na2CO3 (sterilized as a separate solution)
Dose: 5 G_PER_L
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
Trace Elements Solution SL-6
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2378_Natronobacteria_medium (CultureMech:008962)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.423329Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2378 |
| 2026-01-28T19:48:23.612866Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:39.280761+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:01.613936Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008962 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.342000+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronobacteria medium" to "natronobacteria_medium" for consistent matching |
| 2026-05-16T09:03:13.343487+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:36.589607+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.259202+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:26.441262+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.959010+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.651978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8fc54f894f84d0a9541d9ff4c2a7c70b239573e35c747232af79043c1e68bfc9 |