payne_seghal_gibbons_medium_add_agar

CultureMech:008966 bacterial COMPLEX SOLID_AGAR
Original name: Payne, Seghal & Gibbons Medium (add agar)

Overview

External ontology
TOGO:M2381 — Payne, Seghal & Gibbons Medium (add agar)
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2381 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1160.pdf

Composition

flowchart LR
    CultureMech_008966["payne_seghal_gibbons_medium_add_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008966 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008966 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008966 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008966 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_008966 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_008966 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_Trisodium_citrate["Trisodium citrate"]
    CultureMech_008966 --> ing6_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing6_Trisodium_citrate -.-> CHEBI_53258
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_008966 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_agar["agar"]
    CultureMech_008966 --> ing8_agar
    CHEBI_2509["CHEBI:2509"]
    ing8_agar -.-> CHEBI_2509
    ing9_Casamino_acids__Difco_["Casamino acids (Difco)"]
    CultureMech_008966 --> ing9_Casamino_acids__Difco_

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 10 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
250 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.36 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
Role: Buffer; Properties: Organic compound, Simple component, Defined component
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
36 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 agar 20 G_PER_L CHEBI:2509
agar
Role: Solidifying component
10 Casamino acids (Difco) 7.5 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.428509Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2381
2026-01-28T19:48:24.184658Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.636084Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008966
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.367350+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Payne, Seghal & Gibbons Medium (add agar)" to "payne_seghal_gibbons_medium_add_agar" for consistent matching
2026-06-05T07:41:40.956574+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:28.981977+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:05:52.280904+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6c7d4a63e6917befd2d7c7ca8323d84b190ae2323f8fb63c6792f83a5a08cc05