caldivirga_medium

CultureMech:008969 archaea COMPLEX LIQUID
Original name: Caldivirga Medium

Overview

External ontology
TOGO:M2386 — Caldivirga Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2386 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium883.pdf

Composition

flowchart LR
    CultureMech_008969["caldivirga_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008969 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008969 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_008969 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008969 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_008969 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008969 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_008969 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Sulfur__powdered["Sulfur, powdered"]
    CultureMech_008969 --> ing7_Sulfur__powdered
    ing8_Na3_citrate_x_2_H2O["Na3-citrate x 2 H2O"]
    CultureMech_008969 --> ing8_Na3_citrate_x_2_H2O
    CHEBI_32142["CHEBI:32142"]
    ing8_Na3_citrate_x_2_H2O -.-> CHEBI_32142
    ing9_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
    CultureMech_008969 --> ing9_Yeast_extract__OXOID_
    ing10_H2SO4["H2SO4"]
    CultureMech_008969 --> ing10_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing10_H2SO4 -.-> CHEBI_26836
    ing11_N2["N2"]
    CultureMech_008969 --> ing11_N2
    CHEBI_17997["CHEBI:17997"]
    ing11_N2 -.-> CHEBI_17997
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_008969 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Hydrogen_gas["Hydrogen gas"]
    CultureMech_008969 --> ing13_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing13_Hydrogen_gas -.-> CHEBI_18276
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_008969 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_008969 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_008969 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_008969 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_008969 --> ing18_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing18_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing19_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_008969 --> ing19_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing19_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing20_CoSO4["CoSO4"]
    CultureMech_008969 --> ing20_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing20_CoSO4 -.-> CHEBI_53470
    ing21_HCl["HCl"]
    CultureMech_008969 --> ing21_HCl
    CHEBI_17883["CHEBI:17883"]
    ing21_HCl -.-> CHEBI_17883
    ing22_Biotin["Biotin"]
    CultureMech_008969 --> ing22_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing22_Biotin -.-> CHEBI_15956
    ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_008969 --> ing23_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing24_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_008969 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_008969 --> ing25_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_HCl -.-> CHEBI_30961
    ing26_Folic_acid["Folic acid"]
    CultureMech_008969 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_008969 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_Riboflavin["Riboflavin"]
    CultureMech_008969 --> ing28_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing28_Riboflavin -.-> CHEBI_17015
    ing29_Nicotinic_acid["Nicotinic acid"]
    CultureMech_008969 --> ing29_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing29_Nicotinic_acid -.-> CHEBI_15940
    CultureMech_008969 --> more["...2 more"]

Ingredients (32)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
3000.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 1000.0, 1000.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 Sulfur, powdered 10 G_PER_L Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 Na3-citrate x 2 H2O
C6H5O7.2H2O.3Na 294.099 g/mol
2.94 G_PER_L CHEBI:32142
sodium citrate dihydrate
Role: Buffer; Properties: Organic compound, Defined component, Simple component
10 Yeast extract (OXOID) 0.5 G_PER_L Properties: Undefined component, Complex component
11 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
12 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
14 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
Properties: ガス
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
3 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
180 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
5 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
3 G_PER_L CHEBI:87009
vanadyl sulfate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
450 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 CoSO4
Co.O4S 154.996 g/mol
1 G_PER_L CHEBI:53470
cobalt(2+) sulfate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
Role: Solvating media; Properties: Defined component, Solution, Inorganic compound
23 Biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
24 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
5 G_PER_L CHEBI:30753
4-aminobenzoic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
25 Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
5 G_PER_L CHEBI:49105
thiamine hydrochloride
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
26 Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
10 G_PER_L CHEBI:30961
pyridoxine hydrochloride
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
27 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
28 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
29 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
30 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
31 Lipoic acid
C8H14O2S2 206.332 g/mol
5 G_PER_L CHEBI:16494
lipoic acid
Role: Growth factor; Properties: Organic compound, Defined component, Simple component
32 D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
5 G_PER_L CHEBI:31345
Calcium pantothenate
Role: Vitamin; Properties: Defined component

Solutions

Na-resazurin solution (0.1% w/v)

Dose: 0.5 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Allen’s trace element solution

Dose: 10 G_PER_L

Vitamin solution

Dose: 10 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.434468Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2386
2026-01-28T19:48:39.921259Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: HCl
2026-03-09T08:05:07.205303+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:38.919935Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:01.670772Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008969
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.404638+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Caldivirga Medium" to "caldivirga_medium" for consistent matching
2026-05-16T09:03:13.392096+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:36.643396+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:32.276765+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:26.493729+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.975056+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:26.436010+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3f7b2732e56ce8467ae160b6607a465516b03c28b108f3df10ddc6f85da19ec1