Overview
- External ontology
- TOGO:M2391 — Alicyclobacillus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2391 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium402.pdf
Composition
flowchart LR
CultureMech_008974["alicyclobacillus_medium"]
ing0_Distilled_water__for_solid_medium_["Distilled water (for solid medium)"]
CultureMech_008974 --> ing0_Distilled_water__for_solid_medium_
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008974 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008974 --> ing2_Yeast_extract
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_008974 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008974 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_008974 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Glucose["Glucose"]
CultureMech_008974 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Distilled_water["Distilled water"]
CultureMech_008974 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_Agar["Agar"]
CultureMech_008974 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_008974 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_008974 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_008974 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_008974 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_008974 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_008974 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_008974 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Distilled water (for solid medium) | 500 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 8 |
Distilled water
H2O 18.015 g/mol
|
1500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 500.0, 1000.0] |
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A:
Dose: variable VARIABLE
Solution B:
Dose: variable VARIABLE
Solution C:
Dose: variable VARIABLE
Trace element solution SL-6
Dose: 1 G_PER_L
See mediadive_25_Trace_element_solution_SL-6.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2391_Alicyclobacillus_Medium (CultureMech:008974)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.445412Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2391 |
| 2026-01-28T19:48:23.407444Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Solution A:; Added default concentration for ingredient: Solution B:; Added default concentration for ingredient: Solution C: |
| 2026-03-09T08:04:38.914529+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:08.761016Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.723525Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008974 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.460357+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alicyclobacillus Medium" to "alicyclobacillus_medium" for consistent matching |
| 2026-05-16T09:03:13.425288+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.679376+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:26.529848+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.988173+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:11.707111+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7d6ad585ca6a8754766661581f0aab6901c5a746d821810359f888698ec7863f |