Overview
- External ontology
- TOGO:M2399 — Middlebrook 7H10 with OADC
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2399 Original URL: https://www.atcc.org/~/media/E44FDED36A7940E3B4078EA6A49B7199.ashx Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008980["middlebrook_7h10_with_oadc"]
ing0_Glycerol["Glycerol"]
CultureMech_008980 --> ing0_Glycerol
CHEBI_17754["CHEBI:17754"]
ing0_Glycerol -.-> CHEBI_17754
ing1_DI_Water["DI Water"]
CultureMech_008980 --> ing1_DI_Water
ing2_Middlebrook_7H10["Middlebrook 7H10"]
CultureMech_008980 --> ing2_Middlebrook_7H10
ing3_Tryptone["Tryptone"]
CultureMech_008980 --> ing3_Tryptone
ing4_Yeast_extract["Yeast extract"]
CultureMech_008980 --> ing4_Yeast_extract
ing5_Sodium_chloride["Sodium chloride"]
CultureMech_008980 --> ing5_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing5_Sodium_chloride -.-> CHEBI_26710
ing6_NaCl["NaCl"]
CultureMech_008980 --> ing6_NaCl
ing6_NaCl -.-> CHEBI_26710
ing7_Dextrose__BD_215530_["Dextrose (BD 215530)"]
CultureMech_008980 --> ing7_Dextrose__BD_215530_
ing8_Oleic_Acid__Sigma_Cat___O_1008_["Oleic Acid (Sigma Cat # O-1008)"]
CultureMech_008980 --> ing8_Oleic_Acid__Sigma_Cat___O_1008_
ing9_Catalase__beef___Sigma_C_9322_["Catalase (beef) (Sigma C-9322)"]
CultureMech_008980 --> ing9_Catalase__beef___Sigma_C_9322_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glycerol
C3H8O3 92.094 g/mol
|
5 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 2 | DI Water | 1000.0 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] | |
| 3 | Middlebrook 7H10 | 19 G_PER_L | Properties: Defined component, Complex component | |
| 4 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 6 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 | Dextrose (BD 215530) | 2 G_PER_L | Role: Carbon source; Properties: Organic compound, Simple component, Defined component | |
| 9 | Oleic Acid (Sigma Cat # O-1008) | 0.05 G_PER_L | Role: Carbon source; Properties: Organic compound, Simple component, Defined component | |
| 10 | Catalase (beef) (Sigma C-9322) | 0.004 G_PER_L | Properties: Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- middlebrook_7h10_with_oadc (CultureMech:008980)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.458213Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2399 |
| 2026-01-28T19:48:34.222444Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:28.362141Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.779867Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008980 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.521903+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H10 with OADC" to "middlebrook_7h10_with_oadc" for consistent matching |
| 2026-06-05T07:41:33.915646+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.181658+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:45.438133+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4e67e318dcc70661b82374ed6b589f6edf74de68469388ac0482c650bf6b767a |
| 2026-08-15T20:09:10.136421+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |