Overview
- External ontology
- TOGO:M2401 — Middlebrook 7H11 solid medium supplemented with 10% albumin, dextrose, and catalase
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2401 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008984["middlebrook_7h11_solid_medium_supplemented_with_10_albumin_dextrose..."]
ing0_Distilled_water["Distilled water"]
CultureMech_008984 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glycerol["Glycerol"]
CultureMech_008984 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Middlebrook_7H11_solid_medium["Middlebrook 7H11 solid medium"]
CultureMech_008984 --> ing2_Middlebrook_7H11_solid_medium
ing3_Middlebrook_ADC_Growth_Supplement["Middlebrook ADC Growth Supplement"]
CultureMech_008984 --> ing3_Middlebrook_ADC_Growth_Supplement
ing4_Monopotassium_Phosphate["Monopotassium Phosphate"]
CultureMech_008984 --> ing4_Monopotassium_Phosphate
CHEBI_63036["CHEBI:63036"]
ing4_Monopotassium_Phosphate -.-> CHEBI_63036
ing5_Biotin["Biotin"]
CultureMech_008984 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_008984 --> ing6_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing6_Ammonium_Sulfate -.-> CHEBI_62946
ing7_Disodium_Phosphate["Disodium Phosphate"]
CultureMech_008984 --> ing7_Disodium_Phosphate
CHEBI_34683["CHEBI:34683"]
ing7_Disodium_Phosphate -.-> CHEBI_34683
ing8_Magnesium_Sulfate["Magnesium Sulfate"]
CultureMech_008984 --> ing8_Magnesium_Sulfate
CHEBI_32599["CHEBI:32599"]
ing8_Magnesium_Sulfate -.-> CHEBI_32599
ing9_Sodium_Citrate["Sodium Citrate"]
CultureMech_008984 --> ing9_Sodium_Citrate
CHEBI_53258["CHEBI:53258"]
ing9_Sodium_Citrate -.-> CHEBI_53258
ing10_Ferric_Ammonium_Citrate["Ferric Ammonium Citrate"]
CultureMech_008984 --> ing10_Ferric_Ammonium_Citrate
CHEBI_31604["CHEBI:31604"]
ing10_Ferric_Ammonium_Citrate -.-> CHEBI_31604
ing11_L_Glutamic_Acid["L-Glutamic Acid"]
CultureMech_008984 --> ing11_L_Glutamic_Acid
CHEBI_16015["CHEBI:16015"]
ing11_L_Glutamic_Acid -.-> CHEBI_16015
ing12_Pyridoxine["Pyridoxine"]
CultureMech_008984 --> ing12_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing12_Pyridoxine -.-> CHEBI_16709
ing13_Malachite_Green["Malachite Green"]
CultureMech_008984 --> ing13_Malachite_Green
CHEBI_72449["CHEBI:72449"]
ing13_Malachite_Green -.-> CHEBI_72449
ing14_Agar["Agar"]
CultureMech_008984 --> ing14_Agar
CHEBI_2509["CHEBI:2509"]
ing14_Agar -.-> CHEBI_2509
ing15_Casein_Enzymic_Hydrolysate["Casein Enzymic Hydrolysate"]
CultureMech_008984 --> ing15_Casein_Enzymic_Hydrolysate
ing16_Dextrose["Dextrose"]
CultureMech_008984 --> ing16_Dextrose
CHEBI_17634["CHEBI:17634"]
ing16_Dextrose -.-> CHEBI_17634
ing17_Tryptone["Tryptone"]
CultureMech_008984 --> ing17_Tryptone
ing18_Yeast_extract["Yeast extract"]
CultureMech_008984 --> ing18_Yeast_extract
ing19_Sodium_chloride["Sodium chloride"]
CultureMech_008984 --> ing19_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing19_Sodium_chloride -.-> CHEBI_26710
ing20_Catalase["Catalase"]
CultureMech_008984 --> ing20_Catalase
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 450.0, 50.0] |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
2.5 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 3 | Middlebrook 7H11 solid medium | 10.25 G_PER_L | ||
| 4 | Middlebrook ADC Growth Supplement | 50 G_PER_L | ||
| 5 |
Monopotassium Phosphate
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0005 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 7 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Disodium Phosphate
HO4P.2Na 141.958 g/mol
|
1.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Magnesium Sulfate
Mg.O4S 120.368 g/mol
|
0.05 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Sodium Citrate
C6H5O7.3Na 258.069 g/mol
|
0.4 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 11 | Ferric Ammonium Citrate | 0.04 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 12 |
L-Glutamic Acid
C5H9NO4 147.13 g/mol
|
0.5 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 13 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
0.001 G_PER_L |
CHEBI:16709
pyridoxine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
Malachite Green
C23H25N2.Cl 364.92 g/mol
|
0.001 G_PER_L |
CHEBI:72449
malachite green
|
Role: pH indicator, Selective agent; Properties: Organic compound, Defined component, Simple component |
| 15 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 16 | Casein Enzymic Hydrolysate | 1 G_PER_L | Properties: Undefined component | |
| 17 |
Dextrose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 18 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 19 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 20 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 21 | Catalase | 0.0015 G_PER_L | Properties: Simple component, Defined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- middlebrook_7h11_solid_medium_supplemented_with_10_albumin_dextrose_and_catalase (CultureMech:008984)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:56.462214Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2401 |
| 2026-01-28T19:48:11.982264Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:47.670059Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.805846Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008984 |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.549426+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Middlebrook 7H11 solid medium supplemented with 10% albumin, dextrose, and catalase" to "middlebrook_7h11_solid_medium_supplemented_with_10_albumin_dextrose_and_catalase" for consistent matching |
| 2026-06-05T07:41:33.962643+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.228993+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:45.492121+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 20b00ad2f8b8285a8175a279850e4d640cb9bfa712f521d822738dca2a848d4e |