minimal_vitamin_mv_medium

CultureMech:008998 bacterial COMPLEX LIQUID
Original name: minimal vitamin (MV) medium

Overview

External ontology
TOGO:M2415 — minimal vitamin (MV) medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2415

Composition

flowchart LR
    CultureMech_008998["minimal_vitamin_mv_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008998 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
    CultureMech_008998 --> ing1_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4___7H2O -.-> CHEBI_31795
    ing2_CaCl2___2H2O["CaCl2 · 2H2O"]
    CultureMech_008998 --> ing2_CaCl2___2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2___2H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008998 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Na2MoO4___2H2O["Na2MoO4 · 2H2O"]
    CultureMech_008998 --> ing4_Na2MoO4___2H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4___2H2O -.-> CHEBI_75213
    ing5_H3BO3["H3BO3"]
    CultureMech_008998 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008998 --> ing6_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_7H2O -.-> CHEBI_75836
    ing7_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008998 --> ing7_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing7_CoCl2_6H2O -.-> CHEBI_35696
    ing8_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008998 --> ing8_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_7H2O -.-> CHEBI_32312
    ing9_biotin["biotin"]
    CultureMech_008998 --> ing9_biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_biotin -.-> CHEBI_15956
    ing10_thiamine_hydrochloride["thiamine hydrochloride"]
    CultureMech_008998 --> ing10_thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing10_thiamine_hydrochloride -.-> CHEBI_49105
    ing11__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008998 --> ing11__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing11__NH4_2SO4 -.-> CHEBI_62946
    ing12_folic_acid["folic acid"]
    CultureMech_008998 --> ing12_folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_folic_acid -.-> CHEBI_27470
    ing13_vitamin_B12["vitamin B12"]
    CultureMech_008998 --> ing13_vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing13_vitamin_B12 -.-> CHEBI_176843
    ing14_riboflavin["riboflavin"]
    CultureMech_008998 --> ing14_riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_riboflavin -.-> CHEBI_17015
    ing15_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_008998 --> ing15_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_5H2O -.-> CHEBI_31440
    ing16_MnSO4_H2O["MnSO4·H2O"]
    CultureMech_008998 --> ing16_MnSO4_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_H2O -.-> CHEBI_86364
    ing17_nicotinamide["nicotinamide"]
    CultureMech_008998 --> ing17_nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing17_nicotinamide -.-> CHEBI_17154
    ing18_NaH2PO4_H2O["NaH2PO4·H2O"]
    CultureMech_008998 --> ing18_NaH2PO4_H2O
    CHEBI_37585["CHEBI:37585"]
    ing18_NaH2PO4_H2O -.-> CHEBI_37585
    ing19_D_calcium_pantothenate["D-calcium pantothenate"]
    CultureMech_008998 --> ing19_D_calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_D_calcium_pantothenate -.-> CHEBI_31345

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
5.3 MICROG_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 K2HPO4
HO4P.2K 174.174 g/mol
3.8 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 Na2MoO4 · 2H2O
2H2O.MoO4.2Na 241.946 g/mol
47 MICROG_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 H3BO3
H3BO3 61.833 g/mol
30 MICROG_PER_L CHEBI:33118
boric acid
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
2 MG_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 CoCl2·6H2O
2Cl.Co 129.839 g/mol
40 MICROG_PER_L CHEBI:35696
cobalt dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
200 MICROG_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 biotin
C10H16N2O3S 244.316 g/mol
20 MICROG_PER_L CHEBI:15956
biotin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
50 MICROG_PER_L CHEBI:49105
thiamine hydrochloride
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3.6 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
13 folic acid
C19H19N7O6 441.404 g/mol
20 MICROG_PER_L CHEBI:27470
folic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 vitamin B12 1 MICROG_PER_L CHEBI:176843
vitamin B12
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
15 riboflavin
C17H20N4O6 376.369 g/mol
50 MICROG_PER_L CHEBI:17015
riboflavin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
16 CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
40 MICROG_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
200 MICROG_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18 nicotinamide
C6H6N2O 122.127 g/mol
50 MICROG_PER_L CHEBI:17154
nicotinamide
Role: Growth factor; Properties: Organic compound, Defined component, Simple component
19 NaH2PO4·H2O
H2O4P.Na 119.976 g/mol
2.8 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
20 D-calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
50 MICROG_PER_L CHEBI:31345
Calcium pantothenate
Role: Vitamin; Properties: Defined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.483373Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2415
2026-01-28T19:48:10.764024Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.898069Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008998
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.646756+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "minimal vitamin (MV) medium" to "minimal_vitamin_mv_medium" for consistent matching
2026-06-05T07:41:34.804041+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:24.797221+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.081780+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:46.513080+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9695ce45ed218df943fbc39eff8207d79ed9fb27f5bfb6b2e5837de079d31484