Overview
- External ontology
- TOGO:M2421 — Alkaline Nutrient Agar (For sporulation)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2421 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf
Composition
flowchart LR
CultureMech_009004["alkaline_nutrient_agar_for_sporulation"]
ing0_Distilled_water["Distilled water"]
CultureMech_009004 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009004 --> ing1_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing1_MnSO4_x_H2O -.-> CHEBI_86364
ing2_Agar__if_necessary["Agar, if necessary"]
CultureMech_009004 --> ing2_Agar__if_necessary
ing3_Meat_extract["Meat extract"]
CultureMech_009004 --> ing3_Meat_extract
ing4_Peptone["Peptone"]
CultureMech_009004 --> ing4_Peptone
ing5_NaHCO3["NaHCO3"]
CultureMech_009004 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na2CO3_anhydrous["Na2CO3 anhydrous"]
CultureMech_009004 --> ing6_Na2CO3_anhydrous
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3_anhydrous -.-> CHEBI_29377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1100.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1000.0, 100.0] |
| 2 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
10 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 | Agar, if necessary | 15 G_PER_L | Role: Solidifying component | |
| 4 | Meat extract | 3 G_PER_L | Properties: Undefined component | |
| 5 | Peptone | 5 G_PER_L | ||
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Na2CO3 anhydrous
CO3.2Na 105.988 g/mol
|
5.3 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Na-sesquicarbonate solution
Dose: 100 G_PER_L
See mediadive_1174_Na-sesquicarbonate_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaline_nutrient_agar_for_sporulation (CultureMech:009004)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.489488Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2421 |
| 2026-01-28T19:48:09.902583Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:16.083416+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:43.450652Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:01.920468Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009004 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.670660+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Nutrient Agar (For sporulation)" to "alkaline_nutrient_agar_for_sporulation" for consistent matching |
| 2026-05-16T09:03:23.664326+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:47.904851+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.571695+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:22.403642+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5e02c93acc310c08b1a1fd4dc14b30d622030cb5c5d51f7e317d57e45d934a32 |