mrs_agar_slants

CultureMech:009005 bacterial COMPLEX LIQUID
Original name: MRS agar slants

Overview

External ontology
TOGO:M2422 — MRS agar slants
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2422

Composition

flowchart LR
    CultureMech_009005["mrs_agar_slants"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009005 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_009005 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_YE["YE"]
    CultureMech_009005 --> ing2_YE
    ing3_K2HPO4["K2HPO4"]
    CultureMech_009005 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_sodium_acetate["sodium acetate"]
    CultureMech_009005 --> ing4_sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_sodium_acetate -.-> CHEBI_32954
    ing5_CaCO3["CaCO3"]
    CultureMech_009005 --> ing5_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing5_CaCO3 -.-> CHEBI_3311
    ing6_MnSO4_H2O["MnSO4·H2O"]
    CultureMech_009005 --> ing6_MnSO4_H2O
    CHEBI_86364["CHEBI:86364"]
    ing6_MnSO4_H2O -.-> CHEBI_86364
    ing7_triammonium_citrate["triammonium citrate"]
    CultureMech_009005 --> ing7_triammonium_citrate
    CHEBI_63037["CHEBI:63037"]
    ing7_triammonium_citrate -.-> CHEBI_63037
    ing8_glucose["glucose"]
    CultureMech_009005 --> ing8_glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_glucose -.-> CHEBI_17234
    ing9__Lab_Lemco__powder["‘Lab-Lemco’ powder"]
    CultureMech_009005 --> ing9__Lab_Lemco__powder
    ing10_peptone["peptone"]
    CultureMech_009005 --> ing10_peptone

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 YE 4 G_PER_L Role: Nutrient source; Properties: Undefined component
4 K2HPO4
HO4P.2K 174.174 g/mol
2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 sodium acetate
C2H3O2.Na 82.034 g/mol
5 G_PER_L CHEBI:32954
sodium acetate
Role: Buffer; Properties: Organic compound, Defined component, Simple component
6 CaCO3
CO3.Ca 100.086 g/mol
10 G_PER_L CHEBI:3311
calcium carbonate
Role: Protective agent, Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
0.05 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 triammonium citrate
C6H5O7.3H4N 243.216 g/mol
2 G_PER_L CHEBI:63037
triammonium citrate
Role: Nitrogen source; Properties: Simple component, Organic compound, Defined component
9 glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
10 ‘Lab-Lemco’ powder 8 G_PER_L Properties: Undefined component
11 peptone 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.490823Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2422
2026-01-28T19:48:32.317228Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:01.926631Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009005
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:20.676944+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MRS agar slants" to "mrs_agar_slants" for consistent matching
2026-06-05T07:41:38.431311+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:27.272260+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:09.179007+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:50.424612+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint de9c626f1458a62bd17b3e8d7ffef8f16f7f660b6d3f7ee634318dfad2adbc1a