Overview
- External ontology
- TOGO:M243 — Methanothermus Fervidus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M243 Original source: JCM - JCM_M251 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=251
Composition
flowchart LR
CultureMech_009019["methanothermus_fervidus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009019 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_009019 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009019 --> ing2_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing2_Na2S_9H2O -.-> CHEBI_76209
ing3_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009019 --> ing3_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_7H2O -.-> CHEBI_75836
ing4_NaHCO3["NaHCO3"]
CultureMech_009019 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Na2SO4["Na2SO4"]
CultureMech_009019 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6__NH4_2Ni_SO4_2_6H2O["(NH4)2Ni(SO4)2・6H2O"]
CultureMech_009019 --> ing6__NH4_2Ni_SO4_2_6H2O
CHEBI_86149["CHEBI:86149"]
ing6__NH4_2Ni_SO4_2_6H2O -.-> CHEBI_86149
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009019 --> ing7_Yeast_extract__BD_Difco_
ing8_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009019 --> ing8_Trypticase_peptone__BD_BBL_
ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009019 --> ing9_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009019 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_N2["N2"]
CultureMech_009019 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
ing12_Hydrogen_gas["Hydrogen gas"]
CultureMech_009019 --> ing12_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing12_Hydrogen_gas -.-> CHEBI_18276
ing13_K2HPO4["K2HPO4"]
CultureMech_009019 --> ing13_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing13_K2HPO4 -.-> CHEBI_131527
ing14_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009019 --> ing14_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_7H2O -.-> CHEBI_31795
ing15_NaCl["NaCl"]
CultureMech_009019 --> ing15_NaCl
CHEBI_26710["CHEBI:26710"]
ing15_NaCl -.-> CHEBI_26710
ing16_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009019 --> ing16_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing16_CaCl2_2H2O -.-> CHEBI_86158
ing17_KH2PO4["KH2PO4"]
CultureMech_009019 --> ing17_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing17_KH2PO4 -.-> CHEBI_63036
ing18__NH4_2SO4["(NH4)2SO4"]
CultureMech_009019 --> ing18__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing18__NH4_2SO4 -.-> CHEBI_62946
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
922.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 920.0, 1.0, 1.0] |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 3 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
(NH4)2Ni(SO4)2・6H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
3 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 9 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 10 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 14 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NaCl
Cl.Na 58.443 g/mol
|
12 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Mineral solution I (see below)
Dose: 37.5 G_PER_L
Mineral solution II (see below)
Dose: 37.5 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M243_Methanothermus_Fervidus_Medium (CultureMech:009019)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.689201Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M243 |
| 2026-01-28T19:48:30.339787Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:50.416506+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:21.633701Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:01.979870Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009019 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.734116+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanothermus Fervidus Medium" to "methanothermus_fervidus_medium" for consistent matching |
| 2026-05-16T09:03:13.487981+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:36.751235+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:26.595821+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.009777+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.470762+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3a7f414344769e4f3d22c0e65b0eb45d204c92c60301166ccfa4414a9da9645d |