Overview
- External ontology
- TOGO:M246 — Mannitol-Yeast Extract Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M246 Original source: JCM - JCM_M254 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=254
Composition
flowchart LR
CultureMech_009045["mannitol_yeast_extract_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009045 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009045 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009045 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009045 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_009045 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Mannitol["Mannitol"]
CultureMech_009045 --> ing5_Mannitol
CHEBI_29864["CHEBI:29864"]
ing5_Mannitol -.-> CHEBI_29864
ing6_HCl["HCl"]
CultureMech_009045 --> ing6_HCl
CHEBI_17883["CHEBI:17883"]
ing6_HCl -.-> CHEBI_17883
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Mannitol
C6H14O6 182.172 g/mol
|
10 G_PER_L |
CHEBI:29864
mannitol
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mannitol_yeast_extract_medium (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.695290Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M246 |
| 2026-01-28T19:48:31.729750Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-13T06:20:02.193534Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009045 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:20.961310+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mannitol-Yeast Extract Medium" to "mannitol_yeast_extract_medium" for consistent matching |
| 2026-05-16T09:03:40.076256+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:12.690085+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:51.590347+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.835277+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:41.830812+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0774cfa12a787303e4c2dc19c06d1443ecf1cbce3d8c8b0ef9a71c64eaf4b486 |