Overview
- External ontology
- TOGO:M2498 — S medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2498
Composition
flowchart LR
CultureMech_009071["s_medium"]
ing0_distilled_water["distilled water"]
CultureMech_009071 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009071 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCI["NaCI"]
CultureMech_009071 --> ing2_NaCI
ing3_KH2PO4["KH2PO4"]
CultureMech_009071 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_009071 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_009071 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_sodium_succinate["sodium succinate"]
CultureMech_009071 --> ing6_sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_sodium_succinate -.-> CHEBI_63675
ing7_gelatin["gelatin"]
CultureMech_009071 --> ing7_gelatin
CHEBI_5291["CHEBI:5291"]
ing7_gelatin -.-> CHEBI_5291
ing8_Agar_Noble__Difco_["Agar Noble (Difco)"]
CultureMech_009071 --> ing8_Agar_Noble__Difco_
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009071 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_009071 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_009071 --> ing11_MnCl2_x_4_H2O
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009071 --> ing12_CoCl2_x_6_H2O
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009071 --> ing13_NiCl2_x_6_H2O
ing14_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_009071 --> ing14_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_2H2O -.-> CHEBI_86318
ing15_ZnCl2["ZnCl2"]
CultureMech_009071 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_009071 --> ing16_FeCl3_x_6_H2O
ing17_Na2SeO3["Na2SeO3"]
CultureMech_009071 --> ing17_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing17_Na2SeO3 -.-> CHEBI_48843
ing18_EDTA_2Na["EDTA-2Na"]
CultureMech_009071 --> ing18_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing18_EDTA_2Na -.-> CHEBI_64734
ing19_biotin["biotin"]
CultureMech_009071 --> ing19_biotin
CHEBI_15956["CHEBI:15956"]
ing19_biotin -.-> CHEBI_15956
ing20_p_aminobenzoic_acid["p-aminobenzoic acid"]
CultureMech_009071 --> ing20_p_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_aminobenzoic_acid -.-> CHEBI_30753
ing21_thiamine_HCl["thiamine-HCl"]
CultureMech_009071 --> ing21_thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_thiamine_HCl -.-> CHEBI_49105
ing22_pyridoxine_HCl["pyridoxine-HCl"]
CultureMech_009071 --> ing22_pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing22_pyridoxine_HCl -.-> CHEBI_30961
ing23_vitamin_B12["vitamin B12"]
CultureMech_009071 --> ing23_vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing23_vitamin_B12 -.-> CHEBI_176843
ing24_niacin["niacin"]
CultureMech_009071 --> ing24_niacin
CHEBI_15940["CHEBI:15940"]
ing24_niacin -.-> CHEBI_15940
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
102.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | NaCI | 0.2 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 8 | gelatin | 7 G_PER_L |
CHEBI:5291
gelatin
|
Role: Solidifying component; Properties: Undefined component |
| 9 | Agar Noble (Difco) | 12 G_PER_L | Role: Solidifying component; Properties: Defined component | |
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
20 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
100 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | MnCl2 x 4 H2O | 100 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 13 | CoCl2 x 6 H2O | 100 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 14 | NiCl2 x 6 H2O | 10 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
10 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
100 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | FeCl3 x 6 H2O | 2000 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 18 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
5 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
EDTA-2Na
C10H14N2O8.2Na 336.208 g/mol
|
1000 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 20 |
biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
p-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
30 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
50 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 | vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
niacin
C6H5NO2 123.111 g/mol
|
50 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
CaCl2 solution
Dose: 100 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
vitamin solution
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- s_medium (CultureMech:009071)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.586171Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2498 |
| 2026-01-28T19:48:38.659032Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:04.730010+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:36.695494Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:02.362680Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009071 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.137079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "S medium" to "s_medium" for consistent matching |
| 2026-05-16T09:04:31.093686+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:44.962045+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.288436+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:31.631173+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.203274+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.023901+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 598b40506950e27219538fb07dc9f5c1830c3b8fbb0a3f5918bb12f701b7c833 |