Overview
- External ontology
- TOGO:M2518 — LB agar (supplemented with ampicillin)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2518 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009088["lb_agar_supplemented_with_ampicillin"]
ing0_ampicillin["ampicillin"]
CultureMech_009088 --> ing0_ampicillin
CHEBI_28971["CHEBI:28971"]
ing0_ampicillin -.-> CHEBI_28971
ing1_Tryptone["Tryptone"]
CultureMech_009088 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_009088 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_009088 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
ampicillin
C16H19N3O4S 349.412 g/mol
|
150 G_PER_L |
CHEBI:28971
ampicillin
|
Role: Antimicrobial agent; Properties: Defined component, Organic compound, Simple component |
| 2 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 3 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lb_agar_supplemented_with_ampicillin (CultureMech:009088)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.600616Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2518 |
| 2026-01-28T19:48:32.604997Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: LB agar |
| 2026-03-13T06:20:02.423648Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009088 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.197780+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB agar (supplemented with ampicillin)" to "lb_agar_supplemented_with_ampicillin" for consistent matching |
| 2026-05-16T09:03:38.780611+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:10.812007+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:39.971540+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0937fbc4a265aedcb8d23e2ea8c6acbd12ec62bfe7eb39b5be79ab6df89ae355 |