Overview
- External ontology
- TOGO:M2553 — Medium for Nautilia profundicola BAA-1463
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2553 Original URL: https://www.atcc.org/~/media/ED2F144D72B749E9B32F46C366F07F69.ashx
Composition
flowchart LR
CultureMech_009122["medium_for_nautilia_profundicola_baa_1463"]
ing0_NaCl["NaCl"]
CultureMech_009122 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009122 --> ing1_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2___2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_009122 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009122 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Resazurin["Resazurin"]
CultureMech_009122 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2___6H2O["MgCl2 . 6H2O"]
CultureMech_009122 --> ing5_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2___6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_009122 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_So_Sulfur_Powder_["So(Sulfur Powder)"]
CultureMech_009122 --> ing7_So_Sulfur_Powder_
ing8_Na2S["Na2S"]
CultureMech_009122 --> ing8_Na2S
CHEBI_76208["CHEBI:76208"]
ing8_Na2S -.-> CHEBI_76208
ing9_DI_WATER["DI WATER"]
CultureMech_009122 --> ing9_DI_WATER
ing10_Selenite_tungstate["Selenite-tungstate"]
CultureMech_009122 --> ing10_Selenite_tungstate
ing11_Vitamins__ATCC_["Vitamins (ATCC)"]
CultureMech_009122 --> ing11_Vitamins__ATCC_
ing12_2m_HCl["2m HCl"]
CultureMech_009122 --> ing12_2m_HCl
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009122 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Hydrogen_gas["Hydrogen gas"]
CultureMech_009122 --> ing14_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing14_Hydrogen_gas -.-> CHEBI_18276
ing15_Distilled_water["Distilled water"]
CultureMech_009122 --> ing15_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing15_Distilled_water -.-> CHEBI_15377
ing16_Na2MoO4___H2O["Na2MoO4 . H2O"]
CultureMech_009122 --> ing16_Na2MoO4___H2O
CHEBI_75215["CHEBI:75215"]
ing16_Na2MoO4___H2O -.-> CHEBI_75215
ing17_H3BO3["H3BO3"]
CultureMech_009122 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009122 --> ing18_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2___4H2O -.-> CHEBI_86368
ing19_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009122 --> ing19_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing19_CoCl2___6H2O -.-> CHEBI_35696
ing20_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009122 --> ing20_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2___6H2O -.-> CHEBI_34887
ing21_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009122 --> ing21_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4___7H2O -.-> CHEBI_32312
ing22_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009122 --> ing22_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing22_CuCl2___2H2O -.-> CHEBI_86318
ing23_Na2SeO3___5H2O["Na2SeO3 . 5H2O"]
CultureMech_009122 --> ing23_Na2SeO3___5H2O
CHEBI_48843["CHEBI:48843"]
ing23_Na2SeO3___5H2O -.-> CHEBI_48843
ing24_Na2WO4___2H2O["Na2WO4 . 2H2O"]
CultureMech_009122 --> ing24_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4___2H2O -.-> CHEBI_63939
ing25_NaOH["NaOH"]
CultureMech_009122 --> ing25_NaOH
CHEBI_32145["CHEBI:32145"]
ing25_NaOH -.-> CHEBI_32145
ing26_Biotin["Biotin"]
CultureMech_009122 --> ing26_Biotin
CHEBI_15956["CHEBI:15956"]
ing26_Biotin -.-> CHEBI_15956
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009122 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_009122 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_009122 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_009122 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.015 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | So(Sulfur Powder) | 5 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 9 |
Na2S
2Na.S 78.047 g/mol
|
0.2 MOLAR |
CHEBI:76208
sodium sulfide (anhydrous)
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 | DI WATER | 1 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 11 | Selenite-tungstate | 1 G_PER_L | ||
| 12 | Vitamins (ATCC) | 1 G_PER_L | ||
| 13 | 2m HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 15 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 16 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 17 |
Na2MoO4 . H2O
MoO4.2Na 205.916 g/mol
|
0.03 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2SeO3 . 5H2O
2Na.O3Se 172.937 g/mol
|
3 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Na2WO4 . 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 27 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 36 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
100 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
Solutions
NaHCO3 (1M solution)
Dose: 30 G_PER_L
Role: Buffer; Properties: Inorganic compound, Defined component, Solution
Trace Elements (ATCC)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_for_nautilia_profundicola_baa_1463 (CultureMech:009122)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.704354Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2553 |
| 2026-01-28T19:48:12.168589Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 2m HCl; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-02-06T05:10:18.520170Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:20.075660+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:48.028947Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:02.919743Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009122 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.665226+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Nautilia profundicola BAA-1463" to "medium_for_nautilia_profundicola_baa_1463" for consistent matching |
| 2026-05-16T09:04:20.145170+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.245011+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.024586+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:06.109192+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:43.217932+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 83d3c9421ea9bc9241916a5d09ffc9fa1db8db4ce6520737be54d593734c4316 |