Overview
- External ontology
- TOGO:M2554 — Pyrococcus medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2554 Original URL: https://www.atcc.org/~/media/4A37EA717D654CEB9FF4E233DA80040C.ashx
Composition
flowchart LR
CultureMech_009123["pyrococcus_medium"]
ing0_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009123 --> ing0_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing0_Na2S___9H2O -.-> CHEBI_76209
ing1_Powdered_sulfur["Powdered sulfur"]
CultureMech_009123 --> ing1_Powdered_sulfur
ing2_Basal_Medium["Basal Medium"]
CultureMech_009123 --> ing2_Basal_Medium
ing3_KH2PO4["KH2PO4"]
CultureMech_009123 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Resazurin["Resazurin"]
CultureMech_009123 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009123 --> ing5_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing5_NiCl2___6H2O -.-> CHEBI_34887
ing6_Sulfur__powdered["Sulfur, powdered"]
CultureMech_009123 --> ing6_Sulfur__powdered
ing7_Seawater["Seawater"]
CultureMech_009123 --> ing7_Seawater
ing8_Yeast_Extract__BD_212750_["Yeast Extract (BD 212750)"]
CultureMech_009123 --> ing8_Yeast_Extract__BD_212750_
ing9_Peptone__Bacto__BD_211677_["Peptone, Bacto (BD 211677)"]
CultureMech_009123 --> ing9_Peptone__Bacto__BD_211677_
ing10_N2["N2"]
CultureMech_009123 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_Distilled_water["Distilled water"]
CultureMech_009123 --> ing11_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing11_Distilled_water -.-> CHEBI_15377
ing12_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009123 --> ing12_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4___7H2O -.-> CHEBI_31795
ing13_NaCl["NaCl"]
CultureMech_009123 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009123 --> ing14_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4___2H2O -.-> CHEBI_75213
ing15_H3BO3["H3BO3"]
CultureMech_009123 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009123 --> ing16_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4___7H2O -.-> CHEBI_75836
ing17_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009123 --> ing17_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing17_CoCl2___6H2O -.-> CHEBI_35696
ing18_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009123 --> ing18_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4___7H2O -.-> CHEBI_32312
ing19_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009123 --> ing19_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4___5H2O -.-> CHEBI_31440
ing20_AlK_SO4_2___12H2O["AlK(SO4)2 . 12H2O"]
CultureMech_009123 --> ing20_AlK_SO4_2___12H2O
CHEBI_86465["CHEBI:86465"]
ing20_AlK_SO4_2___12H2O -.-> CHEBI_86465
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009123 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_CaCl2["CaCl2"]
CultureMech_009123 --> ing22_CaCl2
CHEBI_3312["CHEBI:3312"]
ing22_CaCl2 -.-> CHEBI_3312
ing23_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009123 --> ing23_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing23_MnSO4___H2O -.-> CHEBI_86364
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
1.0 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.5, 0.5] |
| 2 | Powdered sulfur | 0.18 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 3 | Basal Medium | 6 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Sulfur, powdered | 30 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 8 | Seawater | 1 G_PER_L | Role: Solvating media, Mineral source; Properties: Undefined component | |
| 9 | Yeast Extract (BD 212750) | 1 G_PER_L | Properties: Undefined component | |
| 10 | Peptone, Bacto (BD 211677) | 5 G_PER_L | Properties: Undefined component | |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 12 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
AlK(SO4)2 . 12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 23 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Mineral Solution
Dose: 10 G_PER_L
See mediadive_3952_Wolfe_s_mineral_solution.yaml for composition
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2554_Pyrococcus_medium (CultureMech:009123)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.708505Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2554 |
| 2026-01-28T19:48:07.357617Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:04:11.864508+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:38.651571Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:02.935362Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009123 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.679800+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrococcus medium" to "pyrococcus_medium" for consistent matching |
| 2026-05-16T09:03:14.160000+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.416209+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.143812+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.237105+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.574406+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint acf8ae3a3b21247d8d682e7afc4f6e06117330128891c892e8097c8308fa4abd |