Overview
- External ontology
- TOGO:M2558 — R2 broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2558
Composition
flowchart LR
CultureMech_009127["r2_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_009127 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009127 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_009127 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009127 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_MgSO4["MgSO4"]
CultureMech_009127 --> ing4_MgSO4
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4 -.-> CHEBI_32599
ing5_Glucose["Glucose"]
CultureMech_009127 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_009127 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Casamino_acids["Casamino acids"]
CultureMech_009127 --> ing7_Casamino_acids
ing8_Proteose_peptone["Proteose peptone"]
CultureMech_009127 --> ing8_Proteose_peptone
ing9_N2["N2"]
CultureMech_009127 --> ing9_N2
CHEBI_17997["CHEBI:17997"]
ing9_N2 -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.05 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Casamino acids | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Proteose peptone | 0.5 G_PER_L | Properties: Undefined component | |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- r2_broth (CultureMech:009127)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.728694Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2558 |
| 2026-01-28T19:48:13.458324Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:03.014591Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009127 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.759695+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2 broth" to "r2_broth" for consistent matching |
| 2026-05-16T09:04:30.143075+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:43.554602+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.982012+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.756744+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.063576+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1b2f1170426b2106a300bd2862dbf079502d010a0b92e50a1543f90e23da2d6e |