Overview
- External ontology
- TOGO:M2560 — Modified MG basal medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2560
Composition
flowchart LR
CultureMech_009129["modified_mg_basal_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009129 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009129 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009129 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009129 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2.2H2O"]
CultureMech_009129 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_NH4Cl["NH4Cl"]
CultureMech_009129 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_009129 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgCl2_6H2O["MgCl2.6H2O"]
CultureMech_009129 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_009129 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Vitamins["Vitamins"]
CultureMech_009129 --> ing9_Vitamins
ing10_Na2S_9H2O["Na2S.9H2O"]
CultureMech_009129 --> ing10_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_9H2O -.-> CHEBI_76209
ing11_CO2["CO2"]
CultureMech_009129 --> ing11_CO2
CHEBI_16526["CHEBI:16526"]
ing11_CO2 -.-> CHEBI_16526
ing12_6N_HCl["6N HCl"]
CultureMech_009129 --> ing12_6N_HCl
ing13_Na2MoO4_2H2O["Na2MoO4.2H2O"]
CultureMech_009129 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009129 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_4H2O["MnCl2.4H2O"]
CultureMech_009129 --> ing15_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_4H2O -.-> CHEBI_86368
ing16_CoCl2_6H2O["CoCl2.6H2O"]
CultureMech_009129 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_ZnSO4_7H2O["ZnSO4.7H2O"]
CultureMech_009129 --> ing17_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_7H2O -.-> CHEBI_32312
ing18_CuCl2_2H2O["CuCl2.2H2O"]
CultureMech_009129 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_HCl["HCl"]
CultureMech_009129 --> ing19_HCl
CHEBI_17883["CHEBI:17883"]
ing19_HCl -.-> CHEBI_17883
ing20_Na2WO4_2H2O["Na2WO4.2H2O"]
CultureMech_009129 --> ing20_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_2H2O -.-> CHEBI_63939
ing21_FeSO4_NH4_2SO4_6H2O["FeSO4(NH4)2SO4.6H2O"]
CultureMech_009129 --> ing21_FeSO4_NH4_2SO4_6H2O
ing22_Na2SeO4["Na2SeO4"]
CultureMech_009129 --> ing22_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing22_Na2SeO4 -.-> CHEBI_77775
ing23_NiCl2["NiCl2"]
CultureMech_009129 --> ing23_NiCl2
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2 -.-> CHEBI_34887
ing24_Biotin["Biotin"]
CultureMech_009129 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009129 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009129 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_009129 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_009129 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Riboflavin["Riboflavin"]
CultureMech_009129 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_009129 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
502.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 500.0, 1.0] |
| 2 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3450 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 50 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
18000 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2.2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
150 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
250 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
150 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgCl2.6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4260 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
325 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 | Vitamins | 1 G_PER_L | ||
| 11 |
Na2S.9H2O
9H2O.2Na.S 240.182 g/mol
|
700 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 13 | 6N HCl | variable VARIABLE | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 14 |
Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10.3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
3.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2.4H2O
Cl2Mn.4H2O 197.904 g/mol
|
49.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2.6H2O
2Cl.Co 129.839 g/mol
|
119 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4.7H2O
7H2O.O4S.Zn 287.558 g/mol
|
71.8 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2.2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.85 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2WO4.2H2O
2H2O.2Na.O4W 329.846 g/mol
|
16.5 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 | FeSO4(NH4)2SO4.6H2O | 392 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 23 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
47.3 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
NiCl2
Cl2Ni 129.599 g/mol
|
100 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thiamine
C12H17N4OS 265.362 g/mol
|
5 G_PER_L |
CHEBI:18385
thiamine(1+)
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
5 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace elements
Dose: 1 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_mg_basal_medium (CultureMech:009129)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.730259Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2560 |
| 2026-01-28T19:48:25.469604Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: 6N HCl |
| 2026-03-09T08:04:42.173244+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:12.183133Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:03.033045Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009129 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.777489+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified MG basal medium" to "modified_mg_basal_medium" for consistent matching |
| 2026-05-16T09:04:25.895738+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.304292+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.975993+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:08.795901+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.909921+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dd7814989dfd98d6df36a9926bcf3e9c9472456c07cc029d994fd01f582f1ec0 |