Overview
- External ontology
- TOGO:M2574 — Chopped meat carbohydrates with rumen fluid
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2574 Original URL: https://www.atcc.org/~/media/B26B5E3BE9FE40BE8F851888718FC72C.ashx
Composition
flowchart LR
CultureMech_009143["chopped_meat_carbohydrates_with_rumen_fluid"]
ing0_Starch["Starch"]
CultureMech_009143 --> ing0_Starch
CHEBI_28017["CHEBI:28017"]
ing0_Starch -.-> CHEBI_28017
ing1_Cellobiose["Cellobiose"]
CultureMech_009143 --> ing1_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing1_Cellobiose -.-> CHEBI_17057
ing2_Maltose["Maltose"]
CultureMech_009143 --> ing2_Maltose
CHEBI_18167["CHEBI:18167"]
ing2_Maltose -.-> CHEBI_18167
ing3_Rumen_fluid["Rumen fluid"]
CultureMech_009143 --> ing3_Rumen_fluid
ing4_Glucose["Glucose"]
CultureMech_009143 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_ATCC_Medium_1490["ATCC Medium 1490"]
CultureMech_009143 --> ing5_ATCC_Medium_1490
ing6_Distilled_water["Distilled water"]
CultureMech_009143 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_N_NaOH["N NaOH"]
CultureMech_009143 --> ing7_N_NaOH
ing8_Ground_beef__fat_free_["Ground beef (fat-free)"]
CultureMech_009143 --> ing8_Ground_beef__fat_free_
ing9_0_025__Resazurin["0.025% Resazurin"]
CultureMech_009143 --> ing9_0_025__Resazurin
ing10_Yeast_extract["Yeast extract"]
CultureMech_009143 --> ing10_Yeast_extract
ing11_K2HPO4["K2HPO4"]
CultureMech_009143 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_Agar["Agar"]
CultureMech_009143 --> ing12_Agar
CHEBI_2509["CHEBI:2509"]
ing12_Agar -.-> CHEBI_2509
ing13_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
CultureMech_009143 --> ing13_Trypticase_Peptone__BD_211921_
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009143 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_009143 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_Hydrogen_gas["Hydrogen gas"]
CultureMech_009143 --> ing16_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing16_Hydrogen_gas -.-> CHEBI_18276
ing17_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009143 --> ing17_L_Cysteine___HCl
ing18_95__Ethanol["95% Ethanol"]
CultureMech_009143 --> ing18_95__Ethanol
ing19_Vitamin_K1["Vitamin K1"]
CultureMech_009143 --> ing19_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing19_Vitamin_K1 -.-> CHEBI_18067
ing20_Hemin["Hemin"]
CultureMech_009143 --> ing20_Hemin
CHEBI_50385["CHEBI:50385"]
ing20_Hemin -.-> CHEBI_50385
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Starch | 0.1 PERCENT_W_V |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Defined component, Complex component, Organic compound |
| 2 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.1 PERCENT_W_V |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 |
Maltose
C12H22O11 342.297 g/mol
|
0.1 PERCENT_W_V |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | Rumen fluid | 15 PERCENT_W_V | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.4 PERCENT_W_V |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 6 | ATCC Medium 1490 | 1 G_PER_L | ||
| 7 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 100.0] |
| 8 | N NaOH | 26.0 G_PER_L | Properties: Defined component, Solution, Inorganic compound [Merged 2 duplicates: 25.0, 1.0] | |
| 9 | Ground beef (fat-free) | 500 G_PER_L | Properties: Undefined component | |
| 10 | 0.025% Resazurin | 4 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 11 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 13 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 14 | Trypticase Peptone (BD 211921) | 30 G_PER_L | Properties: Undefined component | |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 18 | L-Cysteine . HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 19 | 95% Ethanol | 30 G_PER_L | Role: Solvating media; Properties: Defined component, Organic compound, Simple component | |
| 20 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.15 G_PER_L |
CHEBI:18067
phylloquinone
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 21 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
50 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Simple component, Organic compound, Defined component |
Solutions
Hemin Solution
Dose: 10 G_PER_L
See mediadive_5229_Hemin_solution.yaml for composition
Vitamin K1 Solution
Dose: 0.2 G_PER_L
See mediadive_2227_Vitamin_K1_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chopped_meat_carbohydrates_with_rumen_fluid (CultureMech:009143)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.772401Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2574 |
| 2026-01-28T19:48:27.201113Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:45.093575+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:15.450166Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:03.223699Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009143 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:21.956226+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped meat carbohydrates with rumen fluid" to "chopped_meat_carbohydrates_with_rumen_fluid" for consistent matching |
| 2026-05-16T09:03:27.364166+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.409139+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.505542+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.548776+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b19170bd8fe6bd95b78ccf6e56b499b6fa97e169c1b0fed777e5bb26800a8237 |