modified_chopped_meat_medium

CultureMech:009145 bacterial COMPLEX SOLID_AGAR
Original name: Modified chopped meat medium

Overview

External ontology
TOGO:M2576 — Modified chopped meat medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2576 Original URL: https://www.atcc.org/~/media/85260BB7A69A4640A5BB1042498807E4.ashx

Composition

flowchart LR
    CultureMech_009145["modified_chopped_meat_medium"]
    ing0_1N_NaOH["1N NaOH"]
    CultureMech_009145 --> ing0_1N_NaOH
    ing1_Distilled_water["Distilled water"]
    CultureMech_009145 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Ground_beef__fat_free_["Ground beef (fat-free)"]
    CultureMech_009145 --> ing2_Ground_beef__fat_free_
    ing3_0_025__Resazurin["0.025% Resazurin"]
    CultureMech_009145 --> ing3_0_025__Resazurin
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_009145 --> ing4_Yeast_extract
    ing5_K2HPO4["K2HPO4"]
    CultureMech_009145 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Agar["Agar"]
    CultureMech_009145 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
    CultureMech_009145 --> ing7_Trypticase_Peptone__BD_211921_
    ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009145 --> ing8_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing8_Carbon_dioxide_gas -.-> CHEBI_16526
    ing9_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009145 --> ing9_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing9_Nitrogen_gas -.-> CHEBI_17997
    ing10_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009145 --> ing10_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing10_Hydrogen_gas -.-> CHEBI_18276
    ing11_L_Cysteine___HCl["L-Cysteine . HCl"]
    CultureMech_009145 --> ing11_L_Cysteine___HCl
    ing12_95__Ethanol["95% Ethanol"]
    CultureMech_009145 --> ing12_95__Ethanol
    ing13_Vitamin_K1["Vitamin K1"]
    CultureMech_009145 --> ing13_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing13_Vitamin_K1 -.-> CHEBI_18067
    ing14_Hemin["Hemin"]
    CultureMech_009145 --> ing14_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing14_Hemin -.-> CHEBI_50385

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 1N NaOH 26.0 G_PER_L Properties: Defined component, Inorganic compound, Solution [Merged 2 duplicates: 25.0, 1.0]
2 Distilled water
H2O 18.015 g/mol
101.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 1.0, 100.0]
3 Ground beef (fat-free) 500 G_PER_L Properties: Undefined component
4 0.025% Resazurin 4 G_PER_L Role: pH dependent redox indicator; Properties: Defined component, Solution
5 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
6 K2HPO4
HO4P.2K 174.174 g/mol
5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
7 Agar 20 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
8 Trypticase Peptone (BD 211921) 30 G_PER_L Properties: Undefined component
9 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
10 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
11 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
12 L-Cysteine . HCl 0.5 G_PER_L Properties: Defined component, Simple component, Organic compound
13 95% Ethanol 30 G_PER_L Role: Solvating media; Properties: Defined component, Organic compound, Simple component
14 Vitamin K1
C31H46O2 450.707 g/mol
0.15 G_PER_L CHEBI:18067
phylloquinone
 Role: Vitamin; Properties: Organic compound, Simple component, Defined component
15 Hemin
C34H32ClFeN4O4 651.952 g/mol
50 G_PER_L CHEBI:50385
hemin
 Role: Growth factor; Properties: Simple component, Organic compound, Defined component

Solutions

Hemin Solution

Dose: 10 G_PER_L

See mediadive_5229_Hemin_solution.yaml for composition

Vitamin K1 Solution

Dose: 0.2 G_PER_L

See mediadive_2227_Vitamin_K1_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.779063Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2576
2026-01-28T19:48:15.798889Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:26.063661+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:54.607757Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:03.250787Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009145
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:21.981138+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified chopped meat medium" to "modified_chopped_meat_medium" for consistent matching
2026-05-16T09:03:14.294599+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:37.557274+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:49.282879+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:12.566180+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 8b1c5dfe8da80c4531c8b0fc6ad6ac641400fb5fb77d0b8035e5cfe2ee8d9ea2