Overview
- External ontology
- TOGO:M2585 — PYG Medium (modified)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2585 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104.pdf
Composition
flowchart LR
CultureMech_009153["pyg_medium_modified"]
ing0_Distilled_water["Distilled water"]
CultureMech_009153 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009153 --> ing1_Yeast_extract
ing2_K2HPO4["K2HPO4"]
CultureMech_009153 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Resazurin["Resazurin"]
CultureMech_009153 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Tween_80["Tween 80"]
CultureMech_009153 --> ing4_Tween_80
CHEBI_53426["CHEBI:53426"]
ing4_Tween_80 -.-> CHEBI_53426
ing5_Glucose["Glucose"]
CultureMech_009153 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Beef_extract["Beef extract"]
CultureMech_009153 --> ing6_Beef_extract
ing7_Trypticase_peptone["Trypticase peptone"]
CultureMech_009153 --> ing7_Trypticase_peptone
ing8_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_009153 --> ing8_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Peptone["Peptone"]
CultureMech_009153 --> ing9_Peptone
ing10_8_N_NaOH["8 N NaOH"]
CultureMech_009153 --> ing10_8_N_NaOH
ing11_CO2["CO2"]
CultureMech_009153 --> ing11_CO2
CHEBI_16526["CHEBI:16526"]
ing11_CO2 -.-> CHEBI_16526
ing12_N2["N2"]
CultureMech_009153 --> ing12_N2
CHEBI_17997["CHEBI:17997"]
ing12_N2 -.-> CHEBI_17997
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009153 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_NaCl["NaCl"]
CultureMech_009153 --> ing14_NaCl
CHEBI_26710["CHEBI:26710"]
ing14_NaCl -.-> CHEBI_26710
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009153 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_KH2PO4["KH2PO4"]
CultureMech_009153 --> ing16_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing16_KH2PO4 -.-> CHEBI_63036
ing17_NaHCO3["NaHCO3"]
CultureMech_009153 --> ing17_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing17_NaHCO3 -.-> CHEBI_32139
ing18_1N_NaOH["1N NaOH"]
CultureMech_009153 --> ing18_1N_NaOH
ing19_Haemin["Haemin"]
CultureMech_009153 --> ing19_Haemin
CHEBI_50385["CHEBI:50385"]
ing19_Haemin -.-> CHEBI_50385
ing20_95__ethanol["95% ethanol"]
CultureMech_009153 --> ing20_95__ethanol
ing21_Vitamin_K1["Vitamin K1"]
CultureMech_009153 --> ing21_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing21_Vitamin_K1 -.-> CHEBI_18067
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2050.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1000.0, 100.0] |
| 2 | Yeast extract | 10 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.0 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 1.0] |
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 5 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Organic compound, Defined component, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | Beef extract | 5 G_PER_L | Properties: Undefined component | |
| 8 | Trypticase peptone | 5 G_PER_L | Properties: Undefined component | |
| 9 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 10 | Peptone | 5 G_PER_L | ||
| 11 | 8 N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 12 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 19 | 1N NaOH | 1 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 20 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
50 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 21 | 95% ethanol | 20 G_PER_L | Role: Solvating media; Properties: Defined component, Organic compound, Simple component | |
| 22 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Salt solution
Dose: 40 G_PER_L
See mediadive_6218_Salt_solution.yaml for composition
Vitamin K1 solution
Dose: 0.2 G_PER_L
See mediadive_2227_Vitamin_K1_solution.yaml for composition
Haemin solution
Dose: 10 G_PER_L
See mediadive_1628_Haemin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2585_PYG_Medium_modified (CultureMech:009153)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.789182Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2585 |
| 2026-01-28T19:48:24.972060Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 8 N NaOH; Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:41.276938+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:11.294005Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:03.307482Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009153 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.035165+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYG Medium (modified)" to "pyg_medium_modified" for consistent matching |
| 2026-05-16T09:03:14.343371+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.609392+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.275568+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.292833+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:12.610532+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6b73be8098c888afe552ea709224e2108339be85b4482e14893ea23648745fa0 |