Overview
- External ontology
- TOGO:M258 — Methanomicrobium Mobile Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M258 Original source: JCM - JCM_M266 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=266 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009157["methanomicrobium_mobile_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009157 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009157 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009157 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009157 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009157 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009157 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_009157 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009157 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009157 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_NaHCO3["NaHCO3"]
CultureMech_009157 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_009157 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009157 --> ing11_Yeast_extract__BD_Difco_
ing12_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009157 --> ing12_Trypticase_peptone__BD_BBL_
ing13_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009157 --> ing13_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing14_Rumen_fluid__clarified__see_below_["Rumen fluid, clarified (see below)"]
CultureMech_009157 --> ing14_Rumen_fluid__clarified__see_below_
ing15_Fatty_acid_mixture__see_below_["Fatty acid mixture (see below)"]
CultureMech_009157 --> ing15_Fatty_acid_mixture__see_below_
ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009157 --> ing16_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing16_Carbon_dioxide_gas -.-> CHEBI_16526
ing17_N2["N2"]
CultureMech_009157 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
ing18_Hydrogen_gas["Hydrogen gas"]
CultureMech_009157 --> ing18_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing18_Hydrogen_gas -.-> CHEBI_18276
ing19_clarified_rumen_fluid["clarified rumen fluid"]
CultureMech_009157 --> ing19_clarified_rumen_fluid
ing20_Valeric_acid["Valeric acid"]
CultureMech_009157 --> ing20_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing20_Valeric_acid -.-> CHEBI_17418
ing21_Isovaleric_acid["Isovaleric acid"]
CultureMech_009157 --> ing21_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing21_Isovaleric_acid -.-> CHEBI_28484
ing22_Isobutyric_acid["Isobutyric acid"]
CultureMech_009157 --> ing22_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing22_Isobutyric_acid -.-> CHEBI_16135
ing23_Tryptone["Tryptone"]
CultureMech_009157 --> ing23_Tryptone
ing24_Yeast_extract["Yeast extract"]
CultureMech_009157 --> ing24_Yeast_extract
ing25_Sodium_chloride["Sodium chloride"]
CultureMech_009157 --> ing25_Sodium_chloride
ing25_Sodium_chloride -.-> CHEBI_26710
ing26_NaOH["NaOH"]
CultureMech_009157 --> ing26_NaOH
CHEBI_32145["CHEBI:32145"]
ing26_NaOH -.-> CHEBI_32145
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
680.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 660.0, 20.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.13 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 13 | Trypticase peptone (BD-BBL) | 1 G_PER_L | Properties: Undefined component | |
| 14 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 15 | Rumen fluid, clarified (see below) | 300 G_PER_L | ||
| 16 | Fatty acid mixture (see below) | 20 G_PER_L | ||
| 17 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 19 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 20 | clarified rumen fluid | variable VARIABLE | Role: Nutrient source; Properties: Undefined component | |
| 21 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:17418
valeric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 22 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:28484
isovaleric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 23 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.5 G_PER_L |
CHEBI:16135
isobutyric acid
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 24 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 25 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 26 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 27 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M258_Methanomicrobium_Mobile_Medium (CultureMech:009157)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.714451Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M258 |
| 2026-01-28T19:48:08.424156Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: clarified rumen fluid; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:13.602030+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:40.591568Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:03.360367Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009157 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.085870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanomicrobium Mobile Medium" to "methanomicrobium_mobile_medium" for consistent matching |
| 2026-05-16T09:03:14.403294+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.669560+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.319387+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.313497+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:26.662781+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f0b7882e244b9df072dc64c58b6c22202f3238827eeb59185f0b1ca362814d52 |