Overview
- External ontology
- TOGO:M2594 — Caldithrix Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2594 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium946a.pdf
Composition
flowchart LR
CultureMech_009161["caldithrix_medium"]
ing0_Sodium_resazurin__0_1__w_v_["Sodium resazurin (0.1% w/v)"]
CultureMech_009161 --> ing0_Sodium_resazurin__0_1__w_v_
ing1_Distilled_water["Distilled water"]
CultureMech_009161 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_KH2PO4["KH2PO4"]
CultureMech_009161 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_009161 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009161 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_Na2CO3["Na2CO3"]
CultureMech_009161 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
CultureMech_009161 --> ing6_Yeast_extract__OXOID_
ing7_Synthetic_seawater__2_x_conc__["Synthetic seawater (2 x conc.)"]
CultureMech_009161 --> ing7_Synthetic_seawater__2_x_conc__
ing8_N2["N2"]
CultureMech_009161 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009161 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009161 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_NaCl["NaCl"]
CultureMech_009161 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009161 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009161 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_009161 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009161 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009161 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009161 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009161 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_009161 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_009161 --> ing20_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_009161 --> ing21_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009161 --> ing22_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009161 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing24_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009161 --> ing24_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing24_MnSO4_x_H2O -.-> CHEBI_86364
ing25_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009161 --> ing25_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing25_MgCl2_x_6_H2O -.-> CHEBI_86345
ing26_KCl["KCl"]
CultureMech_009161 --> ing26_KCl
CHEBI_32588["CHEBI:32588"]
ing26_KCl -.-> CHEBI_32588
ing27_NaBr["NaBr"]
CultureMech_009161 --> ing27_NaBr
CHEBI_63004["CHEBI:63004"]
ing27_NaBr -.-> CHEBI_63004
ing28_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_009161 --> ing28_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing28_SrCl2_x_6_H2O -.-> CHEBI_36385
ing29_KI["KI"]
CultureMech_009161 --> ing29_KI
CHEBI_8346["CHEBI:8346"]
ing29_KI -.-> CHEBI_8346
CultureMech_009161 --> more["...11 more"]
Ingredients (41)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sodium resazurin (0.1% w/v) | 0.5 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
3500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 500.0, 1000.0, 1000.0, 1000.0] |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Yeast extract (OXOID) | 3 G_PER_L | Properties: Undefined component, Complex component | |
| 8 | Synthetic seawater (2 x conc.) | 500 G_PER_L | ||
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
17.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.0, 14.0] |
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
56.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 55.4] |
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.1, 1.5] |
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.06999999999999999 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.01, 0.06] |
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
KI
I.K 166.002 g/mol
|
0.1 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
20 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 32 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
50 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
100 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
50 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 40 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 41 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
50 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
Solutions
Modified Wolin's mineral solution
Dose: 10 G_PER_L
See mediadive_241_Modified_Wolin_s_mineral_solution.yaml for composition
Wolin's vitamin solution (10x)
Dose: 1 G_PER_L
See mediadive_5980_Wolin_s_vitamin_solution_10x.yaml for composition
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2594_Caldithrix_Medium (CultureMech:009161)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.807389Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2594 |
| 2026-01-28T19:48:35.962366Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: KOH solution |
| 2026-02-06T05:10:46.848446Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:59.910327+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:31.699277Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:03.411886Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009161 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.140121+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Caldithrix Medium" to "caldithrix_medium" for consistent matching |
| 2026-05-16T09:03:14.444448+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:37.713448+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:32.775743+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:27.370059+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.329328+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:12.701272+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 57655f2d5a5f266ce97aba1e23b56ce201f1edb6982f86a5e38b2205c49a80a0 |