chopped_meat_medium_atcc_1490_with_formate_and_fumarate_atcc_9733 (CultureMech:009185)

Overview

External ontology

TOGO:M2618 — Chopped Meat Medium (ATCC 1490) with Formate and Fumarate (ATCC 9733)

Applications

Microbial cultivation

Notes

Source: https://togomedium.org/medium/M2618 Original URL: https://www.atcc.org/~/media/78329ED2D2314D3BB1BC2F3BEAA2ECFB.ashx

Composition

flowchart LR
    CultureMech_009185["chopped_meat_medium_atcc_1490_with_formate_and_fumarate_atcc_9733"]
    ing0_ATCC_Medium_1490["ATCC Medium 1490"]
    CultureMech_009185 --> ing0_ATCC_Medium_1490
    ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009185 --> ing1_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing1_Carbon_dioxide_gas -.-> CHEBI_16526
    ing2_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009185 --> ing2_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing2_Nitrogen_gas -.-> CHEBI_17997
    ing3_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009185 --> ing3_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing3_Hydrogen_gas -.-> CHEBI_18276
    ing4_Distilled_water["Distilled water"]
    CultureMech_009185 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_N_NaOH["N NaOH"]
    CultureMech_009185 --> ing5_N_NaOH
    ing6_Ground_beef__fat_free_["Ground beef (fat-free)"]
    CultureMech_009185 --> ing6_Ground_beef__fat_free_
    ing7_0_025__Resazurin["0.025% Resazurin"]
    CultureMech_009185 --> ing7_0_025__Resazurin
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_009185 --> ing8_Yeast_extract
    ing9_K2HPO4["K2HPO4"]
    CultureMech_009185 --> ing9_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing9_K2HPO4 -.-> CHEBI_131527
    ing10_Agar["Agar"]
    CultureMech_009185 --> ing10_Agar
    CHEBI_2509["CHEBI:2509"]
    ing10_Agar -.-> CHEBI_2509
    ing11_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
    CultureMech_009185 --> ing11_Trypticase_Peptone__BD_211921_
    ing12_L_Cysteine___HCl["L-Cysteine . HCl"]
    CultureMech_009185 --> ing12_L_Cysteine___HCl
    ing13_95__Ethanol["95% Ethanol"]
    CultureMech_009185 --> ing13_95__Ethanol
    ing14_Vitamin_K1["Vitamin K1"]
    CultureMech_009185 --> ing14_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing14_Vitamin_K1 -.-> CHEBI_18067
    ing15_Distilled_water_to["Distilled water to"]
    CultureMech_009185 --> ing15_Distilled_water_to
    ing16_Hemin["Hemin"]
    CultureMech_009185 --> ing16_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing16_Hemin -.-> CHEBI_50385
    ing17_DI_Water["DI Water"]
    CultureMech_009185 --> ing17_DI_Water
    ing18_Fumaric_Acid["Fumaric Acid"]
    CultureMech_009185 --> ing18_Fumaric_Acid
    CHEBI_18012["CHEBI:18012"]
    ing18_Fumaric_Acid -.-> CHEBI_18012
    ing19_Sodium_Formate["Sodium Formate"]
    CultureMech_009185 --> ing19_Sodium_Formate
    CHEBI_62965["CHEBI:62965"]
    ing19_Sodium_Formate -.-> CHEBI_62965

Ingredients (20)

#	Ingredient	Concentration	Ontology	Notes
1	ATCC Medium 1490	952.4 G_PER_L
2	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: ガス
3	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
4	Hydrogen gas H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Properties: Gas
5	Distilled water H2O 18.015 g/mol	1 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
6	N NaOH	26.0 G_PER_L		Properties: Defined component, Inorganic compound, Solution [Merged 2 duplicates: 25.0, 1.0]
7	Ground beef (fat-free)	500 G_PER_L		Properties: Undefined component
8	0.025% Resazurin	4 G_PER_L		Role: pH dependent redox indicator; Properties: Defined component, Solution
9	Yeast extract	5 G_PER_L		Role: Nutrient source; Properties: Undefined component
10	K2HPO4 HO4P.2K 174.174 g/mol	5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
11	Agar	20 G_PER_L	CHEBI:2509 agar	Role: Solidifying component
12	Trypticase Peptone (BD 211921)	30 G_PER_L		Properties: Undefined component
13	L-Cysteine . HCl	0.5 G_PER_L		Properties: Organic compound, Simple component, Defined component
14	95% Ethanol	30 G_PER_L		Role: Solvating media; Properties: Defined component, Organic compound, Simple component
15	Vitamin K1 C31H46O2 450.707 g/mol	0.15 G_PER_L	CHEBI:18067 phylloquinone	Role: Vitamin; Properties: Defined component, Simple component, Organic compound
16	Distilled water to	100 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
17	Hemin C34H32ClFeN4O4 651.952 g/mol	50 G_PER_L	CHEBI:50385 hemin	Role: Growth factor; Properties: Simple component, Organic compound, Defined component
18	DI Water	100 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component
19	Fumaric Acid C4H4O4 116.072 g/mol	6 G_PER_L	CHEBI:18012 fumaric acid	Role: Carbon source; Properties: Organic compound, Defind medium, Simple component
20	Sodium Formate CHO2.Na 68.007 g/mol	6 G_PER_L	CHEBI:62965 sodium formate	Properties: Defined component, Simple component, Inorganic compound

Solutions

Formate and Fumarate Solution

Dose: 47.6 G_PER_L

Hemin Solution

Dose: 10 G_PER_L

See mediadive_5229_Hemin_solution.yaml for composition

Vitamin K1 Solution

Dose: 0.2 G_PER_L

See mediadive_2227_Vitamin_K1_solution.yaml for composition

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Bacteroides ureolyticus
Strain
ATCC 33387
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011; EXACT parent-medium match (ATCC 1490 + formate + fumarate). Warning: B. ureolyticus has since been reclassified as Campylobacter ureolyticus, but that is external to this source. | Identifiers: ATCC 33387; reclassified as Campylobacter ureolyticus (external to source)
  Snippet
  Bacteroides ureolyticus ATCC 33387 was grown in ATCC medium 1490 with formate and fumarate. Each species was grown anaerobically without agitation at 37C for 24 or 48 hours.
Preferred term
Parabacteroides distasonis
Term
Id
NCBITaxon:823
Label
Parabacteroides distasonis
Strain
ATCC 8503
Evidence
- Reference
  doi:10.1038/s41598-023-47003-0
  Supports
  SUPPORT
  Explanation
  Kleniewska et al. 2023; grown on ATCC 1490 base (no formate/fumarate stated) -> see variant atcc_1490_base_no_formate_fumarate.
  Snippet
  Parabacteroides distasonis (ATCC 8503) was cultivated in ATCC 1490 Modified Chopped Meat medium at 37C anaerobically.
Preferred term
Fusobacterium nucleatum
Term
Id
NCBITaxon:851
Label
Fusobacterium nucleatum
Strain
ATCC 25586
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011; ATCC 1490 base without formate/fumarate -> see variant atcc_1490_base_no_formate_fumarate.
  Snippet
  Fusobacterium nucleatum ATCC 25586 ... grown in ATCC medium 1490 (Modified chopped meat medium), anaerobically at 37C for 24 or 48 h.
Preferred term
Porphyromonas levii
Term
Id
NCBITaxon:28114
Label
Porphyromonas levii
Strain
ATCC 29147
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011; ATCC 1490 base without formate/fumarate -> see variant atcc_1490_base_no_formate_fumarate.
  Snippet
  Porphyromonas levii ATCC 29147 [was] grown in ATCC medium 1490 (Modified chopped meat medium), anaerobically at 37C for 24 or 48 h.
Preferred term
Prevotella bivia
Term
Id
NCBITaxon:28125
Label
Prevotella bivia
Strain
ATCC 29303
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011; ATCC 1490 base without formate/fumarate -> see variant atcc_1490_base_no_formate_fumarate.
  Snippet
  Prevotella bivia ATCC 29303 ... [was] grown in ATCC medium 1490 (Modified chopped meat medium), anaerobically at 37C for 24 or 48 h.
Preferred term
Lactobacillus crispatus
Term
Id
NCBITaxon:47770
Label
Lactobacillus crispatus
Strain
ATCC 33820
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011; ATCC 1490 base without formate/fumarate -> see variant atcc_1490_base_no_formate_fumarate.
  Snippet
  Lactobacillus crispatus ATCC 33820 was grown in ATCC medium 1490 (Modified chopped meat medium) anaerobically without agitation at 37C for 24 h.
Preferred term
Fusobacterium necrophorum
Term
Id
NCBITaxon:859
Label
Fusobacterium necrophorum
Strain
ATCC 27852
Evidence
- Reference
  doi:10.1002/ptr.765
  Supports
  SUPPORT
  Explanation
  Johnston et al. 2001; carbonate/CO2-buffered ATCC 1490 -> see variant atcc_1490_carbonate_co2_buffered.
  Snippet
  Fusobacterium necrophorum (ATCC 27852) was grown in a modified chopped meat medium (ATCC 1490) buffered with carbonate/CO2; 38C, anaerobic glove bag, serum bottle technique.

Recipe variants

Name
atcc_1490_base_no_formate_fumarate
Relationship
OMITTED_COMPONENT_VARIANT
Description
ATCC medium 1490 (Modified Chopped Meat medium) used WITHOUT the added formate and fumarate of the parent record.
Modifications
- Formate and fumarate omitted relative to the parent (ATCC 1490 + formate + fumarate).
- Base ATCC 1490 Modified Chopped Meat medium; anaerobic, 37C, 24-48 h.
Purpose
Several BV-associated and gut anaerobes are reported on plain ATCC 1490 without the formate/fumarate supplement; model as the base variant rather than asserting growth on the formate+fumarate parent.
Evidence
- Reference
  doi:10.1186/1471-2334-11-200
  Supports
  SUPPORT
  Explanation
  O'Hanlon et al. 2011 distinguishes the plain ATCC 1490 base from the formate+fumarate parent formulation.
  Snippet
  Fusobacterium nucleatum ATCC 25586 and Porphyromonas levii ATCC 29147 were all grown in ATCC medium 1490 (Modified chopped meat medium).
Name
atcc_1490_carbonate_co2_buffered
Relationship
SUPPLEMENTED_VARIANT
Description
ATCC 1490 modified chopped meat medium buffered with carbonate/CO2 (Johnston et al. 2001).
Modifications
- Carbonate/CO2 buffering added to ATCC 1490 base (exact carbonate species/concentration and gas composition not printed).
- For MIC assays, medium clarified through a 0.8 um filter (assay-use processing).
- Anaerobic glove bag; serum bottle technique; 38C.
Purpose
Capture the carbonate/CO2-buffered ATCC 1490 variant used for Fusobacterium necrophorum growth and MIC assays.
Evidence
- Reference
  doi:10.1002/ptr.765
  Supports
  SUPPORT
  Explanation
  Johnston et al. 2001; ATCC 1490-derived variant with carbonate/CO2 buffering; no formate/fumarate, so distinct from the parent.
  Snippet
  Fusobacterium necrophorum (ATCC 27852) was grown in a modified chopped meat medium (ATCC 1490) buffered with carbonate/CO2. For MIC assays, this medium was clarified through a 0.8 um filter.

Provenance

Merged from 1 source record.

chopped_meat_medium_atcc_1490_with_formate_and_fumarate_atcc_9733 (CultureMech:009185)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:56.878926Z	togo-import	Imported from TOGO Medium	Source: TOGO, ID: M2618
2026-01-28T19:48:15.192049Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:25.308235+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 3 solution(s) from ingredients
2026-03-13T03:08:53.817571Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 4 duplicate ingredient(s)
2026-03-13T06:20:03.749759Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:009185
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.477875+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Chopped Meat Medium (ATCC 1490) with Formate and Fumarate (ATCC 9733)" to "chopped_meat_medium_atcc_1490_with_formate_and_fumarate_atcc_9733" for consistent matching
2026-05-16T09:03:27.422823+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-05-28T06:12:21.648981+00:00	deep-research-edison-phase2	ENRICHED	Edison phase-2: Bacteroides ureolyticus ATCC 33387 is an exact parent match; ATCC 1490 base (without formate/fumarate) and carbonate/CO2-buffered ATCC 1490 are variants. [added 7 organism(s), 2 variant(s)]
2026-06-05T07:40:53.479044+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.531236+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T00:58:02.728451+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 7 specific strain(s) named, no community signal (#142)
2026-08-06T03:05:27.622368+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint 54048df36a056bec68d30335d6734a82261bbfb0d371705a6faacc8939966e73