Overview
- External ontology
- TOGO:M2653 — MS medium for methanogens
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2653 Original URL: https://www.atcc.org/~/media/0E4A2A5D877748B9972B492CE502DBCB.ashx
Composition
flowchart LR
CultureMech_009208["ms_medium_for_methanogens"]
ing0_0_2__FeSO4___7H2O["0.2% FeSO4 . 7H2O"]
CultureMech_009208 --> ing0_0_2__FeSO4___7H2O
ing1_0_1__Resazurin["0.1% Resazurin"]
CultureMech_009208 --> ing1_0_1__Resazurin
ing2_25__Sodium_acetate["25% Sodium acetate"]
CultureMech_009208 --> ing2_25__Sodium_acetate
ing3_20__Sodium_formate["20% Sodium formate"]
CultureMech_009208 --> ing3_20__Sodium_formate
ing4_Distilled_water["Distilled water"]
CultureMech_009208 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
ing5_NaHCO3["NaHCO3"]
CultureMech_009208 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Agar["Agar"]
CultureMech_009208 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Cysteine_Sulfide_Reducing_Agent["Cysteine-Sulfide Reducing Agent"]
CultureMech_009208 --> ing7_Cysteine_Sulfide_Reducing_Agent
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009208 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_009208 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
ing10_K2HPO4["K2HPO4"]
CultureMech_009208 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009208 --> ing11_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4___7H2O -.-> CHEBI_31795
ing12_NaCl["NaCl"]
CultureMech_009208 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009208 --> ing13_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2___2H2O -.-> CHEBI_86158
ing14_KH2PO4["KH2PO4"]
CultureMech_009208 --> ing14_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing14_KH2PO4 -.-> CHEBI_63036
ing15__NH4_2SO4["(NH4)2SO4"]
CultureMech_009208 --> ing15__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing15__NH4_2SO4 -.-> CHEBI_62946
ing16_Yeast_extract["Yeast extract"]
CultureMech_009208 --> ing16_Yeast_extract
ing17_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
CultureMech_009208 --> ing17_Trypticase_Peptone__BD_211921_
ing18_N_NaOH["N NaOH"]
CultureMech_009208 --> ing18_N_NaOH
ing19_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009208 --> ing19_L_Cysteine___HCl
ing20_N2["N2"]
CultureMech_009208 --> ing20_N2
ing20_N2 -.-> CHEBI_17997
ing21_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009208 --> ing21_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4___2H2O -.-> CHEBI_75213
ing22_H3BO3["H3BO3"]
CultureMech_009208 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009208 --> ing23_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4___7H2O -.-> CHEBI_75836
ing24_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009208 --> ing24_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing24_CoCl2___6H2O -.-> CHEBI_35696
ing25_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009208 --> ing25_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4___7H2O -.-> CHEBI_32312
ing26_CuSO4___5H2O["CuSO4 . 5H2O"]
CultureMech_009208 --> ing26_CuSO4___5H2O
CHEBI_31440["CHEBI:31440"]
ing26_CuSO4___5H2O -.-> CHEBI_31440
ing27_AlK_SO4_2___12H2O["AlK(SO4)2 . 12H2O"]
CultureMech_009208 --> ing27_AlK_SO4_2___12H2O
CHEBI_86465["CHEBI:86465"]
ing27_AlK_SO4_2___12H2O -.-> CHEBI_86465
ing28_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009208 --> ing28_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing28_Nitrilotriacetic_acid -.-> CHEBI_44557
ing29_CaCl2["CaCl2"]
CultureMech_009208 --> ing29_CaCl2
CHEBI_3312["CHEBI:3312"]
ing29_CaCl2 -.-> CHEBI_3312
CultureMech_009208 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.2% FeSO4 . 7H2O | 0.4 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Solution, Defined component | |
| 2 | 0.1% Resazurin | 0.4 G_PER_L | Role: pH dependent redox indicator; Properties: Solution, Defined component | |
| 3 | 25% Sodium acetate | 4 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Organic compound | |
| 4 | 20% Sodium formate | 6 G_PER_L | Properties: Defined component, Organic compound, Solution | |
| 5 |
Distilled water
H2O 18.015 g/mol
|
773.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 340.0, 1.0, 1.0, 100.0, 330.0, 1.0] |
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Agar | 8 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Cysteine-Sulfide Reducing Agent | 16 G_PER_L | ||
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.6, 3.0] |
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
13.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 12.0, 1.0] |
| 14 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.16 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KH2PO4
H2O4P.K 136.084 g/mol
|
6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | Yeast extract | 20 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 18 | Trypticase Peptone (BD 211921) | 20 G_PER_L | Properties: Undefined component | |
| 19 | N NaOH | 30 G_PER_L | Properties: Defined component, Solution, Inorganic compound | |
| 20 | L-Cysteine . HCl | 5 G_PER_L | Properties: Simple component, Defined component, Organic compound | |
| 21 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 22 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CuSO4 . 5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
AlK(SO4)2 . 12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 30 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Wolfe's Vitamin Solution
Dose: 4 G_PER_L
Role: Growth factor; Properties: Complex component
Mineral Solution #1
Dose: 15 G_PER_L
Mineral Solution #2
Dose: 15 G_PER_L
20% Yeast Extract - Trypticase Solution
Dose: 4 G_PER_L
Wolfe's Mineral Solution
Dose: 4 G_PER_L
See mediadive_3952_Wolfe_s_mineral_solution.yaml for composition
12.5% Na2S stock solution
Dose: 40 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ms_medium_for_methanogens (CultureMech:009208)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.941498Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2653 |
| 2026-01-28T19:48:36.852366Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:05:01.513177+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:33.359781Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:04.051919Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009208 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.779168+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MS medium for methanogens" to "ms_medium_for_methanogens" for consistent matching |
| 2026-05-16T09:04:26.898783+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:38.682387+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:27.452601+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.210608+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.989977+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3bd140e64d76a2e1abe5c4022f4cbfaa88ae9b8c6d095d7f7dd1ff8fd5c01494 |