Overview
- External ontology
- TOGO:M2657 — OSU967
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2657 Original URL: https://www.atcc.org/~/media/1F190C053C274BAA916116889BC765B0.ashx
Composition
flowchart LR
CultureMech_009212["osu967"]
ing0_Distilled_water["Distilled water"]
CultureMech_009212 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009212 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009212 --> ing2_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2___2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_009212 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_009212 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_009212 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2___6H2O["MgCl2 . 6H2O"]
CultureMech_009212 --> ing6_MgCl2___6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2___6H2O -.-> CHEBI_86345
ing7_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009212 --> ing7_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S___9H2O -.-> CHEBI_76209
ing8_NaHCO3["NaHCO3"]
CultureMech_009212 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009212 --> ing9_L_Cysteine___HCl
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009212 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_Nitrogen_gas["Nitrogen gas"]
CultureMech_009212 --> ing11_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing11_Nitrogen_gas -.-> CHEBI_17997
ing12_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009212 --> ing12_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4___2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009212 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl3___4H2O["MnCl3 . 4H2O"]
CultureMech_009212 --> ing14_MnCl3___4H2O
ing15_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009212 --> ing15_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2___6H2O -.-> CHEBI_35696
ing16_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009212 --> ing16_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2___6H2O -.-> CHEBI_34887
ing17_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009212 --> ing17_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2___2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009212 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_FeCl3___6H2O["FeCl3 . 6H2O"]
CultureMech_009212 --> ing19_FeCl3___6H2O
CHEBI_86254["CHEBI:86254"]
ing19_FeCl3___6H2O -.-> CHEBI_86254
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009212 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_Na2SeO4___6H2O["Na2SeO4 . 6H2O"]
CultureMech_009212 --> ing21_Na2SeO4___6H2O
ing22_AlCl3___6H2O["AlCl3 . 6H2O"]
CultureMech_009212 --> ing22_AlCl3___6H2O
CHEBI_30115["CHEBI:30115"]
ing22_AlCl3___6H2O -.-> CHEBI_30115
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1.6 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.6, 1.0] |
| 3 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.16 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.06, 0.1] |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2 . 6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 | L-Cysteine . HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
24 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | MnCl3 . 4H2O | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 16 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
24 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
0.15 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
25 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
FeCl3 . 6H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 22 | Na2SeO4 . 6H2O | 26 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Solution | |
| 23 |
AlCl3 . 6H2O
AlCl3.6H2O 241.431 g/mol
|
0.05 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace Elements
Dose: 10 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- osu967 (CultureMech:009212)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.961623Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2657 |
| 2026-01-28T19:48:23.372588Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:38.823037+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:08.650395Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:04.134369Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009212 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.860167+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "OSU967" to "osu967" for consistent matching |
| 2026-05-16T09:04:28.243376+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:40.644045+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.818279+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.103777+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 76bf3661ac7906333e7d5993aa5777bf6a569fb3460d07c71aeb870b744789d5 |