methanocalculus_medium

CultureMech:009214 archaea COMPLEX LIQUID
Original name: Methanocalculus Medium

Overview

External ontology
TOGO:M265 — Methanocalculus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M265 Original source: JCM - JCM_M272 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=272

Composition

flowchart LR
    CultureMech_009214["methanocalculus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009214 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_009214 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_KH2PO4["KH2PO4"]
    CultureMech_009214 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009214 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Resazurin["Resazurin"]
    CultureMech_009214 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009214 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_NaHCO3["NaHCO3"]
    CultureMech_009214 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Sodium_acetate["Sodium acetate"]
    CultureMech_009214 --> ing7_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate -.-> CHEBI_32954
    ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_009214 --> ing8_Yeast_extract__BD_Difco_
    ing9_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_009214 --> ing9_Trypticase_peptone__BD_BBL_
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009214 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009214 --> ing11_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing11_Hydrogen_gas -.-> CHEBI_18276
    ing12_N2["N2"]
    CultureMech_009214 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 NH4Cl
Cl.H4N 53.492 g/mol
0.9 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
5 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Defined component, Organic compound
6 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.36 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 NaHCO3
CHO3.Na 84.006 g/mol
5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
8 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.5 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
9 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
10 Trypticase peptone (BD-BBL) 2 G_PER_L Properties: Undefined component
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Trace element solution (see Medium [M235])

Dose: 10 G_PER_L

Cross-reference to Medium M235

5% L-Cysteine·HCl·H2O solution

Dose: 10 G_PER_L

5% Na2S·9H2O solution

Dose: 10 G_PER_L

5% Na2S·9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

5% L-Cysteine·HCl·H2O solution

Dose: 10 G_PER_L

Properties: Defined component, Solution, Organic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.727257Z togo-import Imported from TOGO Medium Source: JCM, ID: M265
2026-01-28T19:48:18.003574Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2
2026-03-09T08:04:29.811953+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T03:08:58.701569Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:04.171484Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009214
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:03.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:22.898293+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanocalculus Medium" to "methanocalculus_medium" for consistent matching
2026-05-16T09:04:21.532964+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:30.976392+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.764965+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:29.842804+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b88bc0d0182e5f7fb72040d98645a57a6a2c85f3ec25f58e1bb59e42ea5d55f7