Overview
- External ontology
- TOGO:M2661 — Methanosphaerula (Peat) Medium (for DSM 25616)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2661 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1094.pdf
Composition
flowchart LR
CultureMech_009216["methanosphaerula_peat_medium_for_dsm_25616"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_009216 --> ing0_Yeast_extract
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009216 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Hydrogen_gas["Hydrogen gas"]
CultureMech_009216 --> ing2_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing2_Hydrogen_gas -.-> CHEBI_18276
ing3_Distilled_water["Distilled water"]
CultureMech_009216 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_Nitrogen_gas["Nitrogen gas"]
CultureMech_009216 --> ing4_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing4_Nitrogen_gas -.-> CHEBI_17997
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009216 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009216 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2Mo4_x_2_H2O["Na2Mo4 x 2 H2O"]
CultureMech_009216 --> ing7_Na2Mo4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2Mo4_x_2_H2O -.-> CHEBI_75213
ing8_H3BO3["H3BO3"]
CultureMech_009216 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_009216 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009216 --> ing10_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
ing11_ZnCl2["ZnCl2"]
CultureMech_009216 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009216 --> ing12_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_009216 --> ing13_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing14_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_009216 --> ing14_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing14_MnSO4_x_4_H2O -.-> CHEBI_86358
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_009216 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_Na2_EDTA["Na2-EDTA"]
CultureMech_009216 --> ing16_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing16_Na2_EDTA -.-> CHEBI_64734
ing17_2_N_NaOH["2 N NaOH"]
CultureMech_009216 --> ing17_2_N_NaOH
ing18_N2["N2"]
CultureMech_009216 --> ing18_N2
ing18_N2 -.-> CHEBI_17997
ing19_2__N_Morpholino_ethanesulfonic_acid__MES_["2-(N-Morpholino)ethanesulfonic acid (MES)"]
CultureMech_009216 --> ing19_2__N_Morpholino_ethanesulfonic_acid__MES_
ing20_NaOH["NaOH"]
CultureMech_009216 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
ing21_Biotin["Biotin"]
CultureMech_009216 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009216 --> ing22_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_Aminobenzoic_acid -.-> CHEBI_30753
ing23_Thiamine_HCl["Thiamine-HCl"]
CultureMech_009216 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_009216 --> ing24_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_HCl -.-> CHEBI_30961
ing25_Folic_acid["Folic acid"]
CultureMech_009216 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_009216 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_Riboflavin["Riboflavin"]
CultureMech_009216 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_009216 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_009216 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
CultureMech_009216 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.02 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 3 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 4 |
Distilled water
H2O 18.015 g/mol
|
2940.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 890.0, 1000.0, 20.0, 1000.0, 10.0, 10.0, 10.0] |
| 5 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.56 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.34 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2Mo4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
24 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
19 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
24 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
75 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
9 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
3.45 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
26 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.34 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
37.23 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 18 | 2 N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 19 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 20 | 2-(N-Morpholino)ethanesulfonic acid (MES) | 1.95 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 21 |
NaOH
HO.Na 39.997 g/mol
|
0.2 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Inorganic compound, Simple component, Defined component |
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 31 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component |
| 32 | 2-Mercaptoethanesulfonic acid (Coenzyme M) | 0.08 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 33 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.41 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 34 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
9.6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 932.9 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 12.55 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 20 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 10 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Solution E
Dose: 10 G_PER_L
See mediadive_4959_Solution_E.yaml for composition
Solution F
Dose: 10 G_PER_L
See mediadive_6293_Solution_F.yaml for composition
Solution G
Dose: 10 G_PER_L
See mediadive_3625_Solution_G.yaml for composition
NH4Cl solution (0.1% w/v)
Dose: 26.8 G_PER_L
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Solution
KH2PO4 solution (0.1% w/v)
Dose: 13.6 G_PER_L
Role: Buffer; Properties: Inorganic compound, Solution, Defined component
KCl solution (0.1% w/v)
Dose: 1.5 G_PER_L
Role: Mineral source; Properties: Solution, Defined component
Trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
TRIS-HCl (1.0 M solution, pH 8.0)
Dose: 7.2 G_PER_L
Role: Buffer; Properties: Defined component, Complex component, Solution
NaNTA (0.5 M solution)
Dose: 4.8 G_PER_L
Role: Mineral source; Properties: Organic compound, Defined component, Solution
TiCl3 (15% w/v solution in HCl; Riedel-de Haen)
Dose: 0.55 G_PER_L
Properties: Inorganic compound, Solution, Defined component
NaOH solution
Dose: variable VARIABLE
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanosphaerula_peat_medium_for_dsm_25616 (CultureMech:009216)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.974790Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2661 |
| 2026-01-28T19:48:09.284462Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: 2 N NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: NaOH solution; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:14.998967+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 15 solution(s) from ingredients |
| 2026-03-13T03:08:42.167385Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 12 duplicate ingredient(s) |
| 2026-03-13T06:20:04.212576Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009216 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:22.950233+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methanosphaerula (Peat) Medium (for DSM 25616)" to "methanosphaerula_peat_medium_for_dsm_25616" for consistent matching |
| 2026-05-16T09:04:22.748521+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:32.725145+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.963887+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:58:23.503472+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:07.411117+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:31.433475+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7e3378f28b43bbd5245899190325367a6ae8e0ceac9a03205290a61d661d63ff |