Overview
- External ontology
- TOGO:M2667 — Pyrococcus Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2667 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377.pdf
Composition
flowchart LR
CultureMech_009222["pyrococcus_medium"]
ing0_NiCl2_x_6_H2O__0_1__w_v_["NiCl2 x 6 H2O (0.1% w/v)"]
CultureMech_009222 --> ing0_NiCl2_x_6_H2O__0_1__w_v_
CHEBI_34887["CHEBI:34887"]
ing0_NiCl2_x_6_H2O__0_1__w_v_ -.-> CHEBI_34887
ing1_Distilled_water["Distilled water"]
CultureMech_009222 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_009222 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_009222 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009222 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_009222 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009222 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_009222 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_H3BO3["H3BO3"]
CultureMech_009222 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_KCl["KCl"]
CultureMech_009222 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_009222 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Sulfur__powdered["Sulfur, powdered"]
CultureMech_009222 --> ing11_Sulfur__powdered
ing12_NaBr["NaBr"]
CultureMech_009222 --> ing12_NaBr
CHEBI_63004["CHEBI:63004"]
ing12_NaBr -.-> CHEBI_63004
ing13_Peptone__BD_Bacto_["Peptone (BD Bacto)"]
CultureMech_009222 --> ing13_Peptone__BD_Bacto_
ing14_Yeast_extract__OXOID_["Yeast extract (OXOID)"]
CultureMech_009222 --> ing14_Yeast_extract__OXOID_
ing15_N2_gas["N2 gas"]
CultureMech_009222 --> ing15_N2_gas
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_009222 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_009222 --> ing17_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_009222 --> ing18_NiCl2_x_6_H2O
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_009222 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_009222 --> ing20_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_009222 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_009222 --> ing22_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing22_CoSO4_x_7_H2O -.-> CHEBI_91244
ing23_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
CultureMech_009222 --> ing23_KAl_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing23_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009222 --> ing24_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_009222 --> ing25_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing26_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_009222 --> ing26_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing26_MnSO4_x_H2O -.-> CHEBI_86364
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NiCl2 x 6 H2O (0.1% w/v)
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Solution, Defined component |
| 2 |
Distilled water
H2O 18.015 g/mol
|
2000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1000.0, 1000.0] |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.5, 3.0] |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
14.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 13.85, 1.0] |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.85 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.75, 0.1] |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
15.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 15.0, 0.01] |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
10 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Sulfur, powdered | 30 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 13 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Peptone (BD Bacto) | 5 G_PER_L | Properties: Undefined component | |
| 15 | Yeast extract (OXOID) | 1 G_PER_L | Properties: Undefined component, Complex component | |
| 16 | N2 gas | variable VARIABLE | Properties: Gas | |
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KAl(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 26 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
SrCl2 x 6 H2O solution (0.1% w/v)
Dose: 7 G_PER_L
Role: Mineral source; Properties: Solution, Defined component, Inorganic compound
KI solution (0.01% w/v)
Dose: 0.5 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Citric acid solution (0.1% w/v)
Dose: 5 G_PER_L
Trace element solution
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2667_Pyrococcus_Medium (CultureMech:009222)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.004995Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2667 |
| 2026-01-28T19:48:41.435901Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 gas; Added default concentration for ingredient: KOH solution |
| 2026-03-09T08:05:09.862531+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:41.629584Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:04.346453Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009222 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.084079+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pyrococcus Medium" to "pyrococcus_medium" for consistent matching |
| 2026-05-16T09:03:15.008723+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.285014+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:33.100092+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:27.870877+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.558074+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.040089+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 914552f434d71d75f5e97b2a4a5eb8afb12e3177404b7fdf6bb1a0298544ddfa |