Overview
- External ontology
- TOGO:M2671 — Desulfurococcus medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2671 Original URL: https://www.atcc.org/~/media/AEA2EA84E65F492BB60B2643F34260AC.ashx
Composition
flowchart LR
CultureMech_009227["desulfurococcus_medium"]
ing0_Nitrogen_gas["Nitrogen gas"]
CultureMech_009227 --> ing0_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing0_Nitrogen_gas -.-> CHEBI_17997
ing1_Distilled_water["Distilled water"]
CultureMech_009227 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009227 --> ing2_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4___7H2O -.-> CHEBI_31795
ing3_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009227 --> ing3_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2___2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009227 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009227 --> ing5_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4___2H2O -.-> CHEBI_75213
ing6_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009227 --> ing6_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4___7H2O -.-> CHEBI_75836
ing7_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009227 --> ing7_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2___4H2O -.-> CHEBI_86368
ing8_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009227 --> ing8_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4___7H2O -.-> CHEBI_32312
ing9_CuCl2__2H2O["CuCl2 .2H2O"]
CultureMech_009227 --> ing9_CuCl2__2H2O
CHEBI_86318["CHEBI:86318"]
ing9_CuCl2__2H2O -.-> CHEBI_86318
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_009227 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_CoSO4___7H2O["CoSO4 . 7H2O"]
CultureMech_009227 --> ing11_CoSO4___7H2O
CHEBI_91244["CHEBI:91244"]
ing11_CoSO4___7H2O -.-> CHEBI_91244
ing12_VOSO4___2H2O["VOSO4 . 2H2O"]
CultureMech_009227 --> ing12_VOSO4___2H2O
CHEBI_87009["CHEBI:87009"]
ing12_VOSO4___2H2O -.-> CHEBI_87009
ing13_Na2B4O7___10H2O["Na2B4O7 . 10H2O"]
CultureMech_009227 --> ing13_Na2B4O7___10H2O
CHEBI_131366["CHEBI:131366"]
ing13_Na2B4O7___10H2O -.-> CHEBI_131366
ing14_Sulphur["Sulphur"]
CultureMech_009227 --> ing14_Sulphur
CHEBI_17909["CHEBI:17909"]
ing14_Sulphur -.-> CHEBI_17909
ing15_Yeast_extract["Yeast extract"]
CultureMech_009227 --> ing15_Yeast_extract
ing16_Resazurin["Resazurin"]
CultureMech_009227 --> ing16_Resazurin
CHEBI_8806["CHEBI:8806"]
ing16_Resazurin -.-> CHEBI_8806
ing17_Tryptone__BD_211705_["Tryptone (BD 211705)"]
CultureMech_009227 --> ing17_Tryptone__BD_211705_
ing18_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009227 --> ing18_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing18_Na2S___9H2O -.-> CHEBI_76209
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1300.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 300.0, 450.0, 500.0, 50.0] |
| 3 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
30 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
28 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
220 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CuCl2 .2H2O
Cl2Cu.2H2O 170.482 g/mol
|
50 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoSO4 . 7H2O
Co.7H2O.O4S 281.101 g/mol
|
10 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
VOSO4 . 2H2O
2H2O.O4S.OV 199.036 g/mol
|
30 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2B4O7 . 10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Sulphur
(S)n.S2 96.201 g/mol
|
5 G_PER_L |
CHEBI:17909
polysulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 17 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 18 | Tryptone (BD 211705) | 2 G_PER_L | Properties: Undefined component | |
| 19 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 300 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 450 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 500 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Solution D
Dose: 50 G_PER_L
See mediadive_5313_Solution_D.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2671_Desulfurococcus_medium (CultureMech:009227)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.019140Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2671 |
| 2026-01-28T19:48:16.973797Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:28.062301+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:56.803946Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:20:04.409303Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009227 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.150702+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfurococcus medium" to "desulfurococcus_medium" for consistent matching |
| 2026-05-16T09:03:15.059462+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.339294+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.918468+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.579187+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.079808+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dbcbba457b0d8bb114ca9c6348e9fade5eca66efb4c48d91c3412897e19e07d5 |