Overview
- External ontology
- TOGO:M2677 — Thermoproteus medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2677 Original URL: https://www.atcc.org/~/media/C45C5A59BB764197A28F2DF969186C86.ashx
Composition
flowchart LR
CultureMech_009232["thermoproteus_medium"]
ing0_Nitrogen_gas["Nitrogen gas"]
CultureMech_009232 --> ing0_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing0_Nitrogen_gas -.-> CHEBI_17997
ing1_Hydrogen_gas["Hydrogen gas"]
CultureMech_009232 --> ing1_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing1_Hydrogen_gas -.-> CHEBI_18276
ing2_Distilled_water["Distilled water"]
CultureMech_009232 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009232 --> ing3_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4___7H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_009232 --> ing4_Yeast_extract
ing5_KH2PO4["KH2PO4"]
CultureMech_009232 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Resazurin["Resazurin"]
CultureMech_009232 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009232 --> ing7_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4___7H2O -.-> CHEBI_75836
ing8__NH4_2SO4["(NH4)2SO4"]
CultureMech_009232 --> ing8__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing8__NH4_2SO4 -.-> CHEBI_62946
ing9_CaSO4___2H2O["CaSO4 . 2H2O"]
CultureMech_009232 --> ing9_CaSO4___2H2O
CHEBI_32583["CHEBI:32583"]
ing9_CaSO4___2H2O -.-> CHEBI_32583
ing10_Glucose["Glucose"]
CultureMech_009232 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_Sulfur["Sulfur"]
CultureMech_009232 --> ing11_Sulfur
CHEBI_26833["CHEBI:26833"]
ing11_Sulfur -.-> CHEBI_26833
ing12_Na2S___9H2O["Na2S . 9H2O"]
CultureMech_009232 --> ing12_Na2S___9H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S___9H2O -.-> CHEBI_76209
ing13_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009232 --> ing13_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4___2H2O -.-> CHEBI_75213
ing14_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009232 --> ing14_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2___4H2O -.-> CHEBI_86368
ing15_ZnSO4__7H2O["ZnSO4 .7H2O"]
CultureMech_009232 --> ing15_ZnSO4__7H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4__7H2O -.-> CHEBI_32312
ing16_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009232 --> ing16_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2___2H2O -.-> CHEBI_86318
ing17_CoSO4___7H2O["CoSO4 . 7H2O"]
CultureMech_009232 --> ing17_CoSO4___7H2O
CHEBI_91244["CHEBI:91244"]
ing17_CoSO4___7H2O -.-> CHEBI_91244
ing18_Na2B4O7___10H2O["Na2B4O7 . 10H2O"]
CultureMech_009232 --> ing18_Na2B4O7___10H2O
CHEBI_131366["CHEBI:131366"]
ing18_Na2B4O7___10H2O -.-> CHEBI_131366
ing19_VOSO4___5H2O["VOSO4 . 5H2O"]
CultureMech_009232 --> ing19_VOSO4___5H2O
CHEBI_132758["CHEBI:132758"]
ing19_VOSO4___5H2O -.-> CHEBI_132758
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 2 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 3 |
Distilled water
H2O 18.015 g/mol
|
991.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 490.0, 450.0, 50.0, 1.0] |
| 4 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.462 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.014 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.556 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.264 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
CaSO4 . 2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.344 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
850 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3.6 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
180 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnSO4 .7H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
5 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoSO4 . 7H2O
Co.7H2O.O4S 281.101 g/mol
|
1.2 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2B4O7 . 10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
450 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
VOSO4 . 5H2O
5H2O.O4S.OV 253.081 g/mol
|
3.6 G_PER_L |
CHEBI:132758
vanadyl sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 500 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 450 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 50 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
H2SO4 solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Trace Elements
Dose: 10 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
H2SO4 solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2677_Thermoproteus_medium (CultureMech:009232)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.036471Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2677 |
| 2026-01-28T19:48:42.091678Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 solution; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: H2SO4 solution |
| 2026-03-09T08:05:10.951781+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:42.771080Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:04.486993Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009232 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.229645+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermoproteus medium" to "thermoproteus_medium" for consistent matching |
| 2026-05-16T09:03:15.125638+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.405818+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:27.980496+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.606730+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.155635+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0078092d4630dd0f377bee1621ac0ef490b15619b16b045766256d2fc2690f04 |