Overview
- External ontology
- TOGO:M267 — MJ/Ytct Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M267 Original source: JCM - JCM_M274 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=274
Composition
flowchart LR
CultureMech_009234["mj_ytct_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009234 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_009234 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_Na2CO3["Na2CO3"]
CultureMech_009234 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_NaNO3["NaNO3"]
CultureMech_009234 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_Na2SO4["Na2SO4"]
CultureMech_009234 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_Ferric_citrate["Ferric citrate"]
CultureMech_009234 --> ing5_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing5_Ferric_citrate -.-> CHEBI_144421
ing6_Trisodium_citrate["Trisodium citrate"]
CultureMech_009234 --> ing6_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate -.-> CHEBI_53258
ing7_Sodium_tartrate["Sodium tartrate"]
CultureMech_009234 --> ing7_Sodium_tartrate
CHEBI_63017["CHEBI:63017"]
ing7_Sodium_tartrate -.-> CHEBI_63017
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009234 --> ing8_Yeast_extract__BD_Difco_
ing9_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_009234 --> ing9_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3_5H2O -.-> CHEBI_32150
ing10_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009234 --> ing10_Tryptone__BD_Difco_
ing11_KOH["KOH"]
CultureMech_009234 --> ing11_KOH
CHEBI_32035["CHEBI:32035"]
ing11_KOH -.-> CHEBI_32035
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009234 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_009234 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
40 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
0.25 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.2 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Defined component, Organic compound, Simple component |
| 7 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
1 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 8 |
Sodium tartrate
C4H4O6.2Na 194.05 g/mol
|
1 G_PER_L |
CHEBI:63017
sodium L-tartrate
|
Role: Mineral source; Properties: Organic compound, Simple component, Defined component |
| 9 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Inorganic compound, Simple component, Defined component |
| 11 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 12 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
MJ(-N) synthetic seawater (see Medium [M260])
Dose: 100 G_PER_L
Cross-reference to Medium M260
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M267_MJ_Ytct_Medium (CultureMech:009234)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.732365Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M267 |
| 2026-01-28T19:48:19.508095Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:32.325034+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:04.515564Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009234 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.259819+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ/Ytct Medium" to "mj_ytct_medium" for consistent matching |
| 2026-05-16T09:03:15.137668+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:38.417854+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.613109+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:12.999698+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2a3c423d47599dd3c5b98c85fbc70ede6574482c71b58684235bbd77b36ba111 |