Overview
- External ontology
- TOGO:M2708 — PYE Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2708 Original URL: https://www.atcc.org/~/media/BAF9D822F8104F038F4EF4FF05026487.ashx
Composition
flowchart LR
CultureMech_009259["pye_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009259 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009259 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009259 --> ing2_Yeast_extract
ing3_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009259 --> ing3_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2___2H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_009259 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_009259 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Vitamin_B12["Vitamin B12"]
CultureMech_009259 --> ing6_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing6_Vitamin_B12 -.-> CHEBI_176843
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009259 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
ing8_d_Biotin["d-Biotin"]
CultureMech_009259 --> ing8_d_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_d_Biotin -.-> CHEBI_15956
ing9_Na2S2O3___5H2O["Na2S2O3 . 5H2O"]
CultureMech_009259 --> ing9_Na2S2O3___5H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3___5H2O -.-> CHEBI_32150
ing10_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009259 --> ing10_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4___2H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_009259 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009259 --> ing12_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2___4H2O -.-> CHEBI_86368
ing13_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009259 --> ing13_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2___6H2O -.-> CHEBI_35696
ing14_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009259 --> ing14_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2___6H2O -.-> CHEBI_34887
ing15_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009259 --> ing15_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2___2H2O -.-> CHEBI_86318
ing16_ZnCl2["ZnCl2"]
CultureMech_009259 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_Na2WO4___2H2O["Na2WO4 . 2H2O"]
CultureMech_009259 --> ing17_Na2WO4___2H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4___2H2O -.-> CHEBI_63939
ing18_VOSO4___2H2O["VOSO4 . 2H2O"]
CultureMech_009259 --> ing18_VOSO4___2H2O
CHEBI_87009["CHEBI:87009"]
ing18_VOSO4___2H2O -.-> CHEBI_87009
ing19_EDTA["EDTA"]
CultureMech_009259 --> ing19_EDTA
CHEBI_4735["CHEBI:4735"]
ing19_EDTA -.-> CHEBI_4735
ing20_FeCl3___6H2O["FeCl3 . 6H2O"]
CultureMech_009259 --> ing20_FeCl3___6H2O
CHEBI_86254["CHEBI:86254"]
ing20_FeCl3___6H2O -.-> CHEBI_86254
ing21_Concentrated_HCl["Concentrated HCl"]
CultureMech_009259 --> ing21_Concentrated_HCl
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
252.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 250.0, 1.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
20 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Vitamin B12 | 20 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
2.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 9 |
d-Biotin
C10H16N2O3S 244.316 g/mol
|
15 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Na2S2O3 . 5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Simple component, Inorganic compound, Defined component |
| 11 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.2 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
50 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
5 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
50 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2WO4 . 2H2O
2H2O.2Na.O4W 329.846 g/mol
|
5 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
VOSO4 . 2H2O
2H2O.O4S.OV 199.036 g/mol
|
5 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
EDTA
C10H16N2O8 292.244 g/mol
|
3.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Simple component, Defined component, Organic compound [Merged 2 duplicates: 2.5, 1.0] |
| 21 |
FeCl3 . 6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.5 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 | Concentrated HCl | 1.5 G_PER_L | Role: Solvating media |
Solutions
Trace Elements Solution
Dose: 1 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
Chelated Iron Solution
Dose: 2 G_PER_L
See mediadive_4790_Chelated_iron_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2708_PYE_Medium (CultureMech:009259)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.099475Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2708 |
| 2026-01-28T19:48:31.317107Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:52.171073+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:23.557630Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:04.779215Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009259 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.538472+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYE Medium" to "pye_medium" for consistent matching |
| 2026-05-16T09:03:15.295084+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:38.579318+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:28.130175+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:49.669826+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:13.115987+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a8cbfb155d9b6a24a600d6359cb04089e0846ef64c74ccc96dea4494e50d27fe |