chopped_meat_carbohydrate_medium

CultureMech:009264 bacterial COMPLEX SOLID_AGAR
Original name: Chopped Meat Carbohydrate Medium

Overview

External ontology
TOGO:M2713 — Chopped Meat Carbohydrate Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2713 Original URL: https://www.atcc.org/~/media/D452A885A4FB4443A9A107F6E539FA37.ashx

Composition

flowchart LR
    CultureMech_009264["chopped_meat_carbohydrate_medium"]
    ing0_Yeast_Extract["Yeast Extract"]
    CultureMech_009264 --> ing0_Yeast_Extract
    ing1_K2HPO4["K2HPO4"]
    CultureMech_009264 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_Starch["Starch"]
    CultureMech_009264 --> ing2_Starch
    CHEBI_28017["CHEBI:28017"]
    ing2_Starch -.-> CHEBI_28017
    ing3_Cellobiose["Cellobiose"]
    CultureMech_009264 --> ing3_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing3_Cellobiose -.-> CHEBI_17057
    ing4_Maltose["Maltose"]
    CultureMech_009264 --> ing4_Maltose
    CHEBI_18167["CHEBI:18167"]
    ing4_Maltose -.-> CHEBI_18167
    ing5_Rumen_fluid["Rumen fluid"]
    CultureMech_009264 --> ing5_Rumen_fluid
    ing6_Glucose["Glucose"]
    CultureMech_009264 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Agar["Agar"]
    CultureMech_009264 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Peptone["Peptone"]
    CultureMech_009264 --> ing8_Peptone
    ing9_Chopped_Meat_Broth["Chopped Meat Broth"]
    CultureMech_009264 --> ing9_Chopped_Meat_Broth
    ing10_Cysteine["Cysteine"]
    CultureMech_009264 --> ing10_Cysteine
    CHEBI_15356["CHEBI:15356"]
    ing10_Cysteine -.-> CHEBI_15356
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009264 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_NaOH__1N_["NaOH (1N)"]
    CultureMech_009264 --> ing12_NaOH__1N_
    ing13_DI_Water["DI Water"]
    CultureMech_009264 --> ing13_DI_Water
    ing14_Ground_Beef__fat_free_["Ground Beef (fat-free)"]
    CultureMech_009264 --> ing14_Ground_Beef__fat_free_

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Yeast Extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
2 K2HPO4
HO4P.2K 174.174 g/mol
5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
3 Starch 1 G_PER_L CHEBI:28017
starch
 Role: Carbon source; Properties: Defined component, Organic compound, Complex component
4 Cellobiose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17057
cellobiose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 Maltose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:18167
alpha-maltose
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
6 Rumen fluid 150 G_PER_L Role: Nutrient source; Properties: Undefined component
7 Glucose
C6H12O6 180.156 g/mol
4 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component
8 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
9 Peptone 30 G_PER_L
10 Chopped Meat Broth 850 G_PER_L
11 Cysteine
C3H7NO2S 121.161 g/mol
0.5 G_PER_L CHEBI:15356
cysteine
 Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
13 NaOH (1N) 25 G_PER_L Properties: Defined component, Inorganic compound, Solution
14 DI Water 1000 G_PER_L Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
15 Ground Beef (fat-free) 500 G_PER_L Properties: Undefined component

Solutions

Resazurin Solution (0.025%)

Dose: 4 G_PER_L

Role: pH dependent redox indicator; Properties: Defined component, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.108918Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2713
2026-01-28T19:48:28.005555Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:46.356178+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:04.825401Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009264
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:24.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.587020+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chopped Meat Carbohydrate Medium" to "chopped_meat_carbohydrate_medium" for consistent matching
2026-05-16T09:03:27.330649+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:53.379009+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:54.493972+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:27.515027+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3306fe0c162047663feab40520f6b174ad0ffe5e57ba49898e1f4643910a39e1