Overview
- External ontology
- TOGO:M2716 — PETC medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2716 Original URL: https://www.atcc.org/~/media/3A84F1392FC64621AA73967EEF626E5E.ashx
Composition
flowchart LR
CultureMech_009267["petc_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009267 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
CultureMech_009267 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009267 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_009267 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009267 --> ing4_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2___2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_009267 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_009267 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_KCl["KCl"]
CultureMech_009267 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_009267 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Fructose["Fructose"]
CultureMech_009267 --> ing9_Fructose
CHEBI_28645["CHEBI:28645"]
ing9_Fructose -.-> CHEBI_28645
ing10_Reducing_Agent["Reducing Agent"]
CultureMech_009267 --> ing10_Reducing_Agent
ing11_CO2["CO2"]
CultureMech_009267 --> ing11_CO2
CHEBI_16526["CHEBI:16526"]
ing11_CO2 -.-> CHEBI_16526
ing12_Na2MoO4___2H2O["Na2MoO4 . 2H2O"]
CultureMech_009267 --> ing12_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4___2H2O -.-> CHEBI_75213
ing13_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009267 --> ing13_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2___6H2O -.-> CHEBI_35696
ing14_NiCl2___6H2O["NiCl2 . 6H2O"]
CultureMech_009267 --> ing14_NiCl2___6H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2___6H2O -.-> CHEBI_34887
ing15_ZnSO4___7H2O["ZnSO4 . 7H2O"]
CultureMech_009267 --> ing15_ZnSO4___7H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4___7H2O -.-> CHEBI_32312
ing16_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009267 --> ing16_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2___2H2O -.-> CHEBI_86318
ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009267 --> ing17_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
ing18_Fe_SO4_2_NH4_2___6H2O["Fe(SO4)2(NH4)2 . 6H2O"]
CultureMech_009267 --> ing18_Fe_SO4_2_NH4_2___6H2O
ing19_MnSO4___H2O["MnSO4 . H2O"]
CultureMech_009267 --> ing19_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing19_MnSO4___H2O -.-> CHEBI_86364
ing20_Na2WO4["Na2WO4"]
CultureMech_009267 --> ing20_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing20_Na2WO4 -.-> CHEBI_63940
ing21_Na2SeO4["Na2SeO4"]
CultureMech_009267 --> ing21_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing21_Na2SeO4 -.-> CHEBI_77775
ing22_Biotin["Biotin"]
CultureMech_009267 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009267 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009267 --> ing24_Thiamine___HCl
ing25_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_009267 --> ing25_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_D_____pantothenate -.-> CHEBI_31345
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009267 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_009267 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_009267 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Riboflavin["Riboflavin"]
CultureMech_009267 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_009267 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1082.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 980.0, 1.0, 1.0, 100.0] |
| 2 |
MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.8 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
20 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Fructose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 11 | Reducing Agent | 10 G_PER_L | ||
| 12 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Na2MoO4 . 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
20 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NiCl2 . 6H2O
Cl2Ni 129.599 g/mol
|
20 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
20 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 | Fe(SO4)2(NH4)2 . 6H2O | 0.8 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 20 |
MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2WO4
2Na.O4W 293.816 g/mol
|
20 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
20 G_PER_L |
CHEBI:77775
sodium selenate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 | Thiamine . HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 26 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Thioctic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:30314
(R)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 33 |
Na2S . 9H2O
9H2O.2Na.S 240.182 g/mol
|
4 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
NaOH
HO.Na 39.997 g/mol
|
0.9 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 35 | L-Cysteine . HCl | 4 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 36 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Trace Elements
Dose: 10 G_PER_L
See mediadive_6183_Trace_elements.yaml for composition
Wolfe's Vitamin Solution
Dose: 10 G_PER_L
KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- petc_medium (CultureMech:009267)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.118628Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2716 |
| 2026-01-28T19:48:19.757223Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2; Added default concentration for ingredient: KOH solution; Added default concentration for ingredient: N2 |
| 2026-02-06T05:10:27.743760Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:32.740072+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:02.033685Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:04.877900Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009267 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:23.641431+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PETC medium" to "petc_medium" for consistent matching |
| 2026-05-16T09:04:28.758214+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:41.394931+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.643060+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:58:29.276470+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.049263+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.637779+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint da89ac881bb0003052b9f99fef9704dda303d33f94ea8f890626746120a6e67c |