py_glucose_atcc_medium_1524_with_10_g_glucose_per_liter_of_medium

CultureMech:009274 bacterial COMPLEX LIQUID
Original name: PY-glucose (ATCC medium 1524) with 10 g glucose per liter of medium

Overview

External ontology
TOGO:M2724 — PY-glucose (ATCC medium 1524) with 10 g glucose per liter of medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2724 Original URL: https://www.atcc.org/~/media/3F8B6BDFCC2D44F88C84F6E1FE4DE6A2.ashx

Composition

flowchart LR
    CultureMech_009274["py_glucose_atcc_medium_1524_with_10_g_glucose_per_liter_of_medium"]
    ing0_0_025__Resazurin__aqueous["0.025% Resazurin, aqueous"]
    CultureMech_009274 --> ing0_0_025__Resazurin__aqueous
    ing1_Distilled_water["Distilled water"]
    CultureMech_009274 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009274 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_009274 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Peptone__Bacto__BD_211677_["Peptone, Bacto (BD 211677)"]
    CultureMech_009274 --> ing4_Peptone__Bacto__BD_211677_
    ing5_Trypticase_Peptone__BD_211921_["Trypticase Peptone (BD 211921)"]
    CultureMech_009274 --> ing5_Trypticase_Peptone__BD_211921_
    ing6_L_Cysteine___HCl["L-Cysteine . HCl"]
    CultureMech_009274 --> ing6_L_Cysteine___HCl
    ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009274 --> ing7_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing7_Carbon_dioxide_gas -.-> CHEBI_16526
    ing8_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009274 --> ing8_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing8_Nitrogen_gas -.-> CHEBI_17997
    ing9_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009274 --> ing9_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing9_Hydrogen_gas -.-> CHEBI_18276
    ing10_NaCl["NaCl"]
    CultureMech_009274 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_KH2PO4["KH2PO4"]
    CultureMech_009274 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_K2HPO4["K2HPO4"]
    CultureMech_009274 --> ing12_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing12_K2HPO4 -.-> CHEBI_131527
    ing13_NaHCO3["NaHCO3"]
    CultureMech_009274 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_CaCl2["CaCl2"]
    CultureMech_009274 --> ing14_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing14_CaCl2 -.-> CHEBI_3312
    ing15_MgSO4["MgSO4"]
    CultureMech_009274 --> ing15_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing15_MgSO4 -.-> CHEBI_32599
    ing16_Hemin["Hemin"]
    CultureMech_009274 --> ing16_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing16_Hemin -.-> CHEBI_50385
    ing17_N_NaOH["N NaOH"]
    CultureMech_009274 --> ing17_N_NaOH
    ing18_95__Ethanol["95% Ethanol"]
    CultureMech_009274 --> ing18_95__Ethanol
    ing19_Vitamin_K1["Vitamin K1"]
    CultureMech_009274 --> ing19_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing19_Vitamin_K1 -.-> CHEBI_18067

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 0.025% Resazurin, aqueous 4 G_PER_L Role: pH dependent redox indicator; Properties: Defined component, Solution
2 Distilled water
H2O 18.015 g/mol
1051.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 950.0, 1.0, 100.0]
3 Yeast extract 10 G_PER_L Role: Nutrient source; Properties: Undefined component
4 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
5 Peptone, Bacto (BD 211677) 5 G_PER_L Properties: Undefined component
6 Trypticase Peptone (BD 211921) 5 G_PER_L Properties: Undefined component
7 L-Cysteine . HCl 0.5 G_PER_L Properties: Organic compound, Defined component, Simple component
8 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
9 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
10 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
11 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
12 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
13 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
14 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
15 CaCl2
Ca.2Cl 110.984 g/mol
0.2 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
16 MgSO4
Mg.O4S 120.368 g/mol
0.2 G_PER_L CHEBI:32599
magnesium sulfate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
17 Hemin
C34H32ClFeN4O4 651.952 g/mol
50 G_PER_L CHEBI:50385
hemin
 Role: Growth factor; Properties: Organic compound, Simple component, Defined component
18 N NaOH 1 G_PER_L Properties: Defined component, Inorganic compound, Solution
19 95% Ethanol 30 G_PER_L Role: Solvating media; Properties: Defined component, Organic compound, Simple component
20 Vitamin K1
C31H46O2 450.707 g/mol
0.15 G_PER_L CHEBI:18067
phylloquinone
 Role: Vitamin; Properties: Simple component, Organic compound, Defined component

Solutions

Salt Solution

Dose: 40 G_PER_L

See mediadive_6218_Salt_solution.yaml for composition

Hemin Solution

Dose: 10 G_PER_L

See mediadive_5229_Hemin_solution.yaml for composition

Vitamin K1 Solution

Dose: 0.2 G_PER_L

See mediadive_2227_Vitamin_K1_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.145364Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2724
2026-01-28T19:48:23.102641Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:38.370539+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:08.160036Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:04.989149Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009274
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:23.758619+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PY-glucose (ATCC medium 1524) with 10 g glucose per liter of medium" to "py_glucose_atcc_medium_1524_with_10_g_glucose_per_liter_of_medium" for consistent matching
2026-05-16T09:04:29.718293+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:42.920482+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.542676+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.833543+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint feabddb6db9158cd51006956fba99c16c4df22646a801da50dbbaa0e80d1d9db