Overview
- External ontology
- TOGO:M2753 — Enriched anaerobe medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2753 Original URL: https://www.atcc.org/~/media/0AA3641D26304D6A813BA9419DE0B193.ashx
Composition
flowchart LR
CultureMech_009304["enriched_anaerobe_medium"]
ing0_0_025__Resazurin["0.025% Resazurin"]
CultureMech_009304 --> ing0_0_025__Resazurin
ing1_Distilled_water["Distilled water"]
CultureMech_009304 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Yeast_extract["Yeast extract"]
CultureMech_009304 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_009304 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Hemin["Hemin"]
CultureMech_009304 --> ing4_Hemin
CHEBI_50385["CHEBI:50385"]
ing4_Hemin -.-> CHEBI_50385
ing5_Vitamin_K1["Vitamin K1"]
CultureMech_009304 --> ing5_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing5_Vitamin_K1 -.-> CHEBI_18067
ing6_Agar["Agar"]
CultureMech_009304 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Trypticase["Trypticase"]
CultureMech_009304 --> ing7_Trypticase
ing8_Oxoid_Lab_Lemco_Powder["Oxoid Lab-Lemco Powder"]
CultureMech_009304 --> ing8_Oxoid_Lab_Lemco_Powder
ing9_L_Cysteine___HCl["L-Cysteine . HCl"]
CultureMech_009304 --> ing9_L_Cysteine___HCl
ing10_Ethanol["Ethanol"]
CultureMech_009304 --> ing10_Ethanol
CHEBI_16236["CHEBI:16236"]
ing10_Ethanol -.-> CHEBI_16236
ing11_N_NaOH["N NaOH"]
CultureMech_009304 --> ing11_N_NaOH
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009304 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_009304 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
ing14_Hydrogen_gas["Hydrogen gas"]
CultureMech_009304 --> ing14_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing14_Hydrogen_gas -.-> CHEBI_18276
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.025% Resazurin | 4 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
Hemin
C34H32ClFeN4O4 651.952 g/mol
|
5 G_PER_L |
CHEBI:50385
hemin
|
Role: Growth factor; Properties: Simple component, Organic compound, Defined component |
| 6 |
Vitamin K1
C31H46O2 450.707 g/mol
|
1 G_PER_L |
CHEBI:18067
phylloquinone
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Trypticase | 30 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
Properties: Undefined component |
| 9 | Oxoid Lab-Lemco Powder | 20 G_PER_L | Properties: Undefined component | |
| 10 | L-Cysteine . HCl | 0.5 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 11 |
Ethanol
C2H6O 46.069 g/mol
|
variable VARIABLE |
CHEBI:16236
ethanol
|
Role: Solvating media; Properties: Defined component, Organic compound, Simple component |
| 12 | N NaOH | variable VARIABLE | Properties: Defined component, Inorganic compound, Solution | |
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 15 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- enriched_anaerobe_medium (CultureMech:009304)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.238958Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2753 |
| 2026-01-28T19:48:33.866227Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Ethanol; Added default concentration for ingredient: N NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-13T06:20:05.435949Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009304 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.337297+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Enriched anaerobe medium" to "enriched_anaerobe_medium" for consistent matching |
| 2026-05-16T09:03:31.481686+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:59.579757+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.711574+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/7/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-09-22T00:09:42.934122+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4be0714b4937ef3f0ba902246d6226570bb8c7b8387d5e887ccc82437c02a3bd |