Overview
- External ontology
- TOGO:M2754 — Chopped meat medium with 1% glucose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2754 Original URL: https://www.atcc.org/~/media/0AB039CD914D4A9BAC9CAB709196F694.ashx
Composition
flowchart LR
CultureMech_009305["chopped_meat_medium_with_1_glucose"]
ing0_Distilled_water["Distilled water"]
CultureMech_009305 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_N_NaOH["N NaOH"]
CultureMech_009305 --> ing1_N_NaOH
ing2_Ground_beef__free_of_fat_["Ground beef (free of fat)"]
CultureMech_009305 --> ing2_Ground_beef__free_of_fat_
ing3_Yeast_extract["Yeast extract"]
CultureMech_009305 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_009305 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Glucose["Glucose"]
CultureMech_009305 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Peptone["Peptone"]
CultureMech_009305 --> ing6_Peptone
ing7_L_cysteine___HCl["L-cysteine . HCl"]
CultureMech_009305 --> ing7_L_cysteine___HCl
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_009305 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Hydrogen_gas["Hydrogen gas"]
CultureMech_009305 --> ing9_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing9_Hydrogen_gas -.-> CHEBI_18276
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | N NaOH | 25 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 3 | Ground beef (free of fat) | 500 G_PER_L | Properties: Undefined component | |
| 4 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 7 | Peptone | 30 G_PER_L | ||
| 8 | L-cysteine . HCl | 0.5 G_PER_L | Properties: Simple component, Defined component, Organic compound | |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
0.025% Resazurin solution
Dose: 4 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chopped_meat_medium_with_1_glucose (CultureMech:009305)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.240625Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2754 |
| 2026-01-28T19:48:24.791047Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:41.008400+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:05.442911Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009305 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.344950+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Chopped meat medium with 1% glucose" to "chopped_meat_medium_with_1_glucose" for consistent matching |
| 2026-05-16T09:03:27.457776+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:53.534356+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.555590+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.666457+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3027d743b35fb74992e79af9e0fdfa4fafc87dc3c1d51a440ade0de6fcff4b19 |