Overview
- External ontology
- TOGO:M2760 — Phosphate-buffered basal medium (Pbbm)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2760
Composition
flowchart LR
CultureMech_009311["phosphate_buffered_basal_medium_pbbm"]
ing0_Resazurin__0_02__["Resazurin (0.02%)"]
CultureMech_009311 --> ing0_Resazurin__0_02__
ing1_Na2S___9H2O__2_5__["Na2S . 9H2O (2.5%)"]
CultureMech_009311 --> ing1_Na2S___9H2O__2_5__
ing2_Distilled_water["Distilled water"]
CultureMech_009311 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Yeast_extract["Yeast extract"]
CultureMech_009311 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_009311 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2___2H2O["CaCl2 . 2H2O"]
CultureMech_009311 --> ing5_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2___2H2O -.-> CHEBI_86158
ing6_NH4Cl["NH4Cl"]
CultureMech_009311 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Glucose["Glucose"]
CultureMech_009311 --> ing7_Glucose
CHEBI_17234["CHEBI:17234"]
ing7_Glucose -.-> CHEBI_17234
ing8_MgSO4___2H2O["MgSO4 . 2H2O"]
CultureMech_009311 --> ing8_MgSO4___2H2O
ing9_Trace_mineral["Trace mineral"]
CultureMech_009311 --> ing9_Trace_mineral
ing10_Phosphate_buffer__1_M__pH_7_2_["Phosphate buffer (1 M, pH 7.2)"]
CultureMech_009311 --> ing10_Phosphate_buffer__1_M__pH_7_2_
ing11_H3BO3["H3BO3"]
CultureMech_009311 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_FeSO4___7H2O["FeSO4 . 7H2O"]
CultureMech_009311 --> ing12_FeSO4___7H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4___7H2O -.-> CHEBI_75836
ing13_MnCl2___4H2O["MnCl2 . 4H2O"]
CultureMech_009311 --> ing13_MnCl2___4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2___4H2O -.-> CHEBI_86368
ing14_CoCl2___6H2O["CoCl2 . 6H2O"]
CultureMech_009311 --> ing14_CoCl2___6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2___6H2O -.-> CHEBI_35696
ing15_CuCl2___2H2O["CuCl2 . 2H2O"]
CultureMech_009311 --> ing15_CuCl2___2H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2___2H2O -.-> CHEBI_86318
ing16_ZnCl2["ZnCl2"]
CultureMech_009311 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_NTA["NTA"]
CultureMech_009311 --> ing17_NTA
CHEBI_39054["CHEBI:39054"]
ing17_NTA -.-> CHEBI_39054
ing18_Na2SeO3["Na2SeO3"]
CultureMech_009311 --> ing18_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing18_Na2SeO3 -.-> CHEBI_48843
ing19_NiSO4___6H2O["NiSO4 . 6H2O"]
CultureMech_009311 --> ing19_NiSO4___6H2O
CHEBI_53001["CHEBI:53001"]
ing19_NiSO4___6H2O -.-> CHEBI_53001
ing20_Na2MoO3["Na2MoO3"]
CultureMech_009311 --> ing20_Na2MoO3
ing21_Biotin["Biotin"]
CultureMech_009311 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_p_aminobenzoic_acid["p-aminobenzoic acid"]
CultureMech_009311 --> ing22_p_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing22_p_aminobenzoic_acid -.-> CHEBI_30753
ing23_Thiamine___HCl["Thiamine . HCl"]
CultureMech_009311 --> ing23_Thiamine___HCl
ing24_Pyridoxine___HCl["Pyridoxine . HCl"]
CultureMech_009311 --> ing24_Pyridoxine___HCl
ing25_Folic_acid["Folic acid"]
CultureMech_009311 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Cyanocobalamine["Cyanocobalamine"]
CultureMech_009311 --> ing26_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing26_Cyanocobalamine -.-> CHEBI_17439
ing27_Riboflavin["Riboflavin"]
CultureMech_009311 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_009311 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Pantothenic_acid["Pantothenic acid"]
CultureMech_009311 --> ing29_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing29_Pantothenic_acid -.-> CHEBI_7916
CultureMech_009311 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Resazurin (0.02%) | 1 G_PER_L | Role: pH dependent redox indicator; Properties: Defined component, Solution | |
| 2 | Na2S . 9H2O (2.5%) | 25 G_PER_L | Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
946.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 944.0, 1.0, 1.0] |
| 4 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
1.9 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.9, 1.0] |
| 6 |
CaCl2 . 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.30000000000000004 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.2, 0.1] |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Glucose
C6H12O6 180.156 g/mol
|
20 MILLIMOLAR |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 9 | MgSO4 . 2H2O | 0.2 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 10 | Trace mineral | 10 G_PER_L | ||
| 11 | Phosphate buffer (1 M, pH 7.2) | 10 G_PER_L | ||
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2 . 4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2 . 6H2O
2Cl.Co 129.839 g/mol
|
0.17 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CuCl2 . 2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | NTA | 1.5 G_PER_L |
CHEBI:39054
NTA
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 19 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.017 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NiSO4 . 6H2O
Ni.O4S 154.756 g/mol
|
0.026 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 | Na2MoO3 | 0.01 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
p-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 | Thiamine . HCl | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 25 | Pyridoxine . HCl | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
0.1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
5 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 32 | 1M KH2PO4 | 10 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound | |
| 33 | 1M Na2HPO4 | 10 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound |
Solutions
Vitamin solution
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- phosphate_buffered_basal_medium_pbbm (CultureMech:009311)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.254432Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2760 |
| 2026-01-28T19:48:24.027517Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:39.707102+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:09.585181Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:05.517091Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009311 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.424503+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Phosphate-buffered basal medium (Pbbm)" to "phosphate_buffered_basal_medium_pbbm" for consistent matching |
| 2026-05-16T09:04:29.022293+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:41.779056+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.190013+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.889801+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c8ed55e0f095a209c88b278f6234856a9801727f41446fc239ec3e5305d8a0e6 |