Overview
- External ontology
- TOGO:M2773 — Serpulina Murdochii Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2773 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium840.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_009321["serpulina_murdochii_medium"]
ing0_sterile_bovine_serum__heat_inactivated_at_56_C_for_45_min_["sterile bovine serum (heat inactivated at 56°C for 45 min)"]
CultureMech_009321 --> ing0_sterile_bovine_serum__heat_inactivated_at_56_C_for_45_min_
ing1_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_009321 --> ing1_Pancreatic_digest_of_casein
ing2_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_009321 --> ing2_Peptic_digest_of_soybean_meal
ing3_Glucose["Glucose"]
CultureMech_009321 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_009321 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_009321 --> ing5_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing5_Dipotassium_phosphate -.-> CHEBI_131527
ing6_Agar["Agar"]
CultureMech_009321 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_air["air"]
CultureMech_009321 --> ing7_air
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009321 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_009321 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | sterile bovine serum (heat inactivated at 56°C for 45 min) | 0.35 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 3 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 4 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 8 | air | 0.5 G_PER_L | Properties: ガス | |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
sterile 10% w/v NaHCO3 solution
Dose: 0.3 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
sterile 30% w/v glucose solution
Dose: 0.05 G_PER_L
Role: Carbon source; Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- TOGO_M2773_Serpulina_Murdochii_Medium (CultureMech:009321)
- TOGO_M2774_Serpulina_Murdochii_Medium (CultureMech:009322)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.275729Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2773 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:42.008990+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:20:05.606909Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009321 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T19:46:24.515417+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Serpulina Murdochii Medium" to "serpulina_murdochii_medium" for consistent matching |
| 2026-05-16T09:03:15.996495+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-08-06T03:05:13.872366+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: TOGO_M2773_Serpulina_Murdochii_Medium, TOGO_M2774_Serpulina_Murdochii_Medium |
| 2026-08-06T03:05:13.872401+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint ccf063ace22be9157b5c37da25226433bd43b4a073e0e4eee08d032c9a075e5f |