Overview
- External ontology
- TOGO:M2775 — Sphaerochaeta Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2775 Original URL: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1204.pdf
Composition
flowchart LR
CultureMech_009323["sphaerochaeta_medium"]
ing0_Destilled_water["Destilled water"]
CultureMech_009323 --> ing0_Destilled_water
ing1_Yeast_extract["Yeast extract"]
CultureMech_009323 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009323 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_009323 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009323 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009323 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_009323 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_009323 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Na2CO3["Na2CO3"]
CultureMech_009323 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na2SO4["Na2SO4"]
CultureMech_009323 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_Maltose_monohydrate["Maltose monohydrate"]
CultureMech_009323 --> ing10_Maltose_monohydrate
ing11_Caseine_peptone["Caseine peptone"]
CultureMech_009323 --> ing11_Caseine_peptone
ing12_DL_Dithiothreitol__DTT_["DL-Dithiothreitol (DTT)"]
CultureMech_009323 --> ing12_DL_Dithiothreitol__DTT_
ing13_N2["N2"]
CultureMech_009323 --> ing13_N2
CHEBI_17997["CHEBI:17997"]
ing13_N2 -.-> CHEBI_17997
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009323 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Destilled water | 1000 G_PER_L | Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component | |
| 2 | Yeast extract | 4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.15 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 11 | Maltose monohydrate | 2 G_PER_L | Role: Carbon source; Properties: Organic compound, Simple component, Defined component | |
| 12 | Caseine peptone | 2 G_PER_L | Properties: Undefined component | |
| 13 | DL-Dithiothreitol (DTT) | 0.8 G_PER_L | Properties: Defined component, Simple component, Inorganic compound | |
| 14 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
Solutions
Na-resazurin solution (0.1% w/v)
Dose: 0.5 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M2775_Sphaerochaeta_Medium (CultureMech:009323)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.277859Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2775 |
| 2026-01-28T19:48:39.717509Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas |
| 2026-03-09T08:05:06.809211+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:05.618603Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009323 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.526927+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sphaerochaeta Medium" to "sphaerochaeta_medium" for consistent matching |
| 2026-05-16T09:03:16.017574+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:39.277349+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:49.928969+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:13.884076+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5982e3a6b065b9377ffc412d551745d180ca7cca69b05d27a42ed82699029564 |