clostridial_growth_medium_cgm

CultureMech:009327 bacterial COMPLEX LIQUID
Original name: Clostridial Growth Medium (CGM)

Overview

External ontology
TOGO:M2779 — Clostridial Growth Medium (CGM)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M2779

Composition

flowchart LR
    CultureMech_009327["clostridial_growth_medium_cgm"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009327 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4___7H2O["MgSO4 . 7H2O"]
    CultureMech_009327 --> ing1_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4___7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_009327 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_009327 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009327 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_FeSO4___7H2O["FeSO4 . 7H2O"]
    CultureMech_009327 --> ing5_FeSO4___7H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4___7H2O -.-> CHEBI_75836
    ing6_ZnSO4___7H2O["ZnSO4 . 7H2O"]
    CultureMech_009327 --> ing6_ZnSO4___7H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4___7H2O -.-> CHEBI_32312
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009327 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_CaCl2["CaCl2"]
    CultureMech_009327 --> ing8_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing8_CaCl2 -.-> CHEBI_3312
    ing9_MnSO4___H2O["MnSO4 . H2O"]
    CultureMech_009327 --> ing9_MnSO4___H2O
    CHEBI_86364["CHEBI:86364"]
    ing9_MnSO4___H2O -.-> CHEBI_86364
    ing10_Na2SeO3["Na2SeO3"]
    CultureMech_009327 --> ing10_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing10_Na2SeO3 -.-> CHEBI_48843
    ing11_CoCl2["CoCl2"]
    CultureMech_009327 --> ing11_CoCl2
    CHEBI_35696["CHEBI:35696"]
    ing11_CoCl2 -.-> CHEBI_35696
    ing12_Glucose["Glucose"]
    CultureMech_009327 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_Tryptone["Tryptone"]
    CultureMech_009327 --> ing13_Tryptone

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 FeSO4 . 7H2O
Fe.7H2O.O4S 278.013 g/mol
0.015 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 ZnSO4 . 7H2O
7H2O.O4S.Zn 287.558 g/mol
0.002 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
9 CaCl2
Ca.2Cl 110.984 g/mol
0.01 G_PER_L CHEBI:3312
calcium dichloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 MnSO4 . H2O
H2O.Mn.O4S 169.016 g/mol
0.01 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
11 Na2SeO3
2Na.O3Se 172.937 g/mol
0.00025 G_PER_L CHEBI:48843
disodium selenite
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
12 CoCl2
2Cl.Co 129.839 g/mol
0.002 G_PER_L CHEBI:35696
cobalt dichloride
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
13 Glucose
C6H12O6 180.156 g/mol
50 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Defined component, Organic compound, Simple component
14 Tryptone 2 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.287929Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2779
2026-01-28T19:48:23.281417Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:05.659989Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009327
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.570478+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Clostridial Growth Medium (CGM)" to "clostridial_growth_medium_cgm" for consistent matching
2026-06-05T07:40:53.652717+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:39.271124+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:54.599710+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:27.762391+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 66d02591439a02df2e6b046ecb3bab9630977b744523c51e68c85db278734151