ema_medium

CultureMech:009335 bacterial COMPLEX LIQUID
Original name: EMA medium

Overview

External ontology
TOGO:M2788 — EMA medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M2788

Composition

flowchart LR
    CultureMech_009335["ema_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009335 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Acid_hydrolyzed_casein["Acid-hydrolyzed casein"]
    CultureMech_009335 --> ing1_Acid_hydrolyzed_casein
    ing2_50x_Vogel_Bonner_salts_["50x Vogel-Bonner salts "]
    CultureMech_009335 --> ing2_50x_Vogel_Bonner_salts_
    ing3_D_Glucose["D-Glucose"]
    CultureMech_009335 --> ing3_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_D_Glucose -.-> CHEBI_17634
    ing4_Nicotinic_acid["Nicotinic acid"]
    CultureMech_009335 --> ing4_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing4_Nicotinic_acid -.-> CHEBI_15940
    ing5_Pantothenic_acid["Pantothenic acid"]
    CultureMech_009335 --> ing5_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing5_Pantothenic_acid -.-> CHEBI_7916
    ing6_MgSO4___7H2O["MgSO4 . 7H2O"]
    CultureMech_009335 --> ing6_MgSO4___7H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4___7H2O -.-> CHEBI_31795
    ing7_K2HPO4__anhydrous_["K2HPO4 (anhydrous)"]
    CultureMech_009335 --> ing7_K2HPO4__anhydrous_
    ing8_NaNH4HPO4___4H2O["NaNH4HPO4 . 4H2O"]
    CultureMech_009335 --> ing8_NaNH4HPO4___4H2O
    ing9_Citric_acid___H2O["Citric acid . H2O"]
    CultureMech_009335 --> ing9_Citric_acid___H2O
    CHEBI_31404["CHEBI:31404"]
    ing9_Citric_acid___H2O -.-> CHEBI_31404

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
981.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 980.0, 1.0]
2 Acid-hydrolyzed casein 1 G_PER_L Properties: Undefined component
3 50x Vogel-Bonner salts 20 G_PER_L
4 D-Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17634
D-glucose
Role: Carbon source; Properties: Simple component, Defined component, Organic compound
5 Nicotinic acid
C6H5NO2 123.111 g/mol
10 G_PER_L CHEBI:15940
nicotinic acid
Role: Vitamin; Properties: Defined component, Simple component, Organic compound
6 Pantothenic acid
C9H17NO5 219.237 g/mol
10 G_PER_L CHEBI:7916
pantothenic acid
Role: Vitamin; Properties: Simple component, Organic compound, Defined component
7 MgSO4 . 7H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
8 K2HPO4 (anhydrous) 500 G_PER_L Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
9 NaNH4HPO4 . 4H2O 175 G_PER_L Role: Mineral source; Properties: Simple component, Defined component
10 Citric acid . H2O
C6H8O7.H2O 210.138 g/mol
100 G_PER_L CHEBI:31404
Citric acid monohydrate
Properties: Defined component, Simple component, Organic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.300486Z togo-import Imported from TOGO Medium Source: TOGO, ID: M2788
2026-01-28T19:48:34.298104Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:28.501246Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:20:05.714567Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009335
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:24.630096+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EMA medium" to "ema_medium" for consistent matching
2026-06-05T07:40:59.501821+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:43.023194+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:05:33.519219+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 58b685df3837ccad31210fa8c0714184d4c709c862b709a0308be640e8ee00dc