Overview
- External ontology
- TOGO:M2789 — M2SGC broth containing tetracycline (10 μg/ml) or rifampin (100 μg/ml)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2789
Composition
flowchart LR
CultureMech_009336["m2sgc_broth_containing_tetracycline_10_g_ml_or_rifampin_100_g_ml"]
ing0_Tetracycline["Tetracycline"]
CultureMech_009336 --> ing0_Tetracycline
ing1_M2SGC_medium["M2SGC medium"]
CultureMech_009336 --> ing1_M2SGC_medium
ing2_CO2["CO2"]
CultureMech_009336 --> ing2_CO2
CHEBI_16526["CHEBI:16526"]
ing2_CO2 -.-> CHEBI_16526
ing3_Distilled_water["Distilled water"]
CultureMech_009336 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009336 --> ing4_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_7H2O -.-> CHEBI_31795
ing5_Yeast_extract["Yeast extract"]
CultureMech_009336 --> ing5_Yeast_extract
ing6_NaCl["NaCl"]
CultureMech_009336 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_KH2PO4["KH2PO4"]
CultureMech_009336 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_K2HPO4["K2HPO4"]
CultureMech_009336 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_Resazurin["Resazurin"]
CultureMech_009336 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_NaHCO3["NaHCO3"]
CultureMech_009336 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_009336 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_CaCl2["CaCl2"]
CultureMech_009336 --> ing12_CaCl2
CHEBI_3312["CHEBI:3312"]
ing12_CaCl2 -.-> CHEBI_3312
ing13_Cellobiose["Cellobiose"]
CultureMech_009336 --> ing13_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing13_Cellobiose -.-> CHEBI_17057
ing14_Cysteine["Cysteine"]
CultureMech_009336 --> ing14_Cysteine
CHEBI_15356["CHEBI:15356"]
ing14_Cysteine -.-> CHEBI_15356
ing15_Clarified_rumen_fluid["Clarified rumen fluid"]
CultureMech_009336 --> ing15_Clarified_rumen_fluid
ing16_Glucose["Glucose"]
CultureMech_009336 --> ing16_Glucose
CHEBI_17234["CHEBI:17234"]
ing16_Glucose -.-> CHEBI_17234
ing17_Soluble_starch["Soluble starch"]
CultureMech_009336 --> ing17_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing17_Soluble_starch -.-> CHEBI_28017
ing18_Casitone["Casitone"]
CultureMech_009336 --> ing18_Casitone
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tetracycline | 10 G_PER_L | Role: Antimicrobial agent; Properties: Defined component, Simple component | |
| 2 | M2SGC medium | 100 G_PER_L | ||
| 3 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 4 |
Distilled water
H2O 18.015 g/mol
|
100 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.009 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 | Yeast extract | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.09 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.045 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.045 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.09 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.009 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.2 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 15 |
Cysteine
C3H7NO2S 121.161 g/mol
|
0.1 G_PER_L |
CHEBI:15356
cysteine
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 16 | Clarified rumen fluid | 30 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 17 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 18 | Soluble starch | 0.2 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 19 | Casitone | 1 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- m2sgc_broth_containing_tetracycline_10_g_ml_or_rifampin_100_g_ml (CultureMech:009336)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.302012Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2789 |
| 2026-01-28T19:48:41.851380Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: CO2 |
| 2026-03-13T06:20:05.723002Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009336 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.638517+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M2SGC broth containing tetracycline (10 μg/ml) or rifampin (100 μg/ml)" to "m2sgc_broth_containing_tetracycline_10_g_ml_or_rifampin_100_g_ml" for consistent matching |
| 2026-05-16T09:03:39.481846+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:11.823443+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.692288+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.901035+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2133af473e424d7ceec4736c3a0c9b2342be047a430cee01716da2975fbbb98e |