Overview
- External ontology
- TOGO:M2809 — LS4D
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2809
Composition
flowchart LR
CultureMech_009357["ls4d"]
ing0_Distilled_water["Distilled water"]
CultureMech_009357 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NH4Cl["NH4Cl"]
CultureMech_009357 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Na2SO4["Na2SO4"]
CultureMech_009357 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2["CaCl2"]
CultureMech_009357 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_009357 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_MgCl2["MgCl2"]
CultureMech_009357 --> ing5_MgCl2
CHEBI_6636["CHEBI:6636"]
ing5_MgCl2 -.-> CHEBI_6636
ing6_PIPES_buffer["PIPES buffer"]
CultureMech_009357 --> ing6_PIPES_buffer
ing7_K2PO4["K2PO4"]
CultureMech_009357 --> ing7_K2PO4
ing8_NaOH["NaOH"]
CultureMech_009357 --> ing8_NaOH
CHEBI_32145["CHEBI:32145"]
ing8_NaOH -.-> CHEBI_32145
ing9_Thauers_vitamins["Thauers vitamins"]
CultureMech_009357 --> ing9_Thauers_vitamins
ing10_Trace_minerals["Trace minerals"]
CultureMech_009357 --> ing10_Trace_minerals
ing11_Titanium_citrate["Titanium citrate"]
CultureMech_009357 --> ing11_Titanium_citrate
ing12_Biotin["Biotin"]
CultureMech_009357 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_009357 --> ing13_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
ing14_Thiamine_hydrochloride["Thiamine hydrochloride"]
CultureMech_009357 --> ing14_Thiamine_hydrochloride
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_hydrochloride -.-> CHEBI_49105
ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_009357 --> ing15_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing16_Folic_acid["Folic acid"]
CultureMech_009357 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_009357 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_Riboflavin["Riboflavin"]
CultureMech_009357 --> ing18_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing18_Riboflavin -.-> CHEBI_17015
ing19_Nicotinic_acid["Nicotinic acid"]
CultureMech_009357 --> ing19_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing19_Nicotinic_acid -.-> CHEBI_15940
ing20_Choline_chloride["Choline chloride"]
CultureMech_009357 --> ing20_Choline_chloride
CHEBI_133341["CHEBI:133341"]
ing20_Choline_chloride -.-> CHEBI_133341
ing21_DL_Pantothenic_acid["DL-Pantothenic acid"]
CultureMech_009357 --> ing21_DL_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing21_DL_Pantothenic_acid -.-> CHEBI_7916
ing22_Thioctic_acid__lipoic_acid_["Thioctic acid (lipoic acid)"]
CultureMech_009357 --> ing22_Thioctic_acid__lipoic_acid_
ing23_N2["N2"]
CultureMech_009357 --> ing23_N2
CHEBI_17997["CHEBI:17997"]
ing23_N2 -.-> CHEBI_17997
ing24_Na2MoO4_2H2O["Na2MoO4.2H2O"]
CultureMech_009357 --> ing24_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_2H2O -.-> CHEBI_75213
ing25_H3BO3["H3BO3"]
CultureMech_009357 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
ing26_MnCl2_4H2O["MnCl2.4H2O"]
CultureMech_009357 --> ing26_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing26_MnCl2_4H2O -.-> CHEBI_86368
ing27_CoCl2_6H2O["CoCl2.6H2O"]
CultureMech_009357 --> ing27_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing27_CoCl2_6H2O -.-> CHEBI_35696
ing28_ZnCl2["ZnCl2"]
CultureMech_009357 --> ing28_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing28_ZnCl2 -.-> CHEBI_49976
ing29_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009357 --> ing29_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing29_Nitrilotriacetic_acid -.-> CHEBI_44557
CultureMech_009357 --> more["...5 more"]
Ingredients (35)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
20 MILLIMOLAR |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
50 MILLIMOLAR |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.6 MILLIMOLAR |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
60 MILLIMOLAR |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2
2Cl.Mg 95.211 g/mol
|
8 MILLIMOLAR |
CHEBI:6636
magnesium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | PIPES buffer | 30 MILLIMOLAR | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 8 | K2PO4 | 2.2 MILLIMOLAR | Role: Buffer; Properties: Inorganic compound, Defined component, Complex component, Solution | |
| 9 |
NaOH
HO.Na 39.997 g/mol
|
10 MILLIMOLAR |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 10 | Thauers vitamins | 1 G_PER_L | ||
| 11 | Trace minerals | 12.5 G_PER_L | ||
| 12 | Titanium citrate | 5 G_PER_L | ||
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Choline chloride
C5H14NO.Cl 139.626 g/mol
|
200 G_PER_L |
CHEBI:133341
choline chloride
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 22 |
DL-Pantothenic acid
C9H17NO5 219.237 g/mol
|
5 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 | Thioctic acid (lipoic acid) | 5 G_PER_L | Role: Growth factor; Properties: Organic compound, Defined component, Simple component | |
| 24 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 25 |
Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
50 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
20 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
MnCl2.4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
CoCl2.6H2O
2Cl.Co 129.839 g/mol
|
0.3 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.2 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 31 |
FeCl2.4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 | 8% (wt/vol) Na2CO3 | 100 G_PER_L | Role: Buffer; Properties: Defined component, Inorganic compound, Solution | |
| 33 | 0.2 M Sodium citrate | 500 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Organic compound | |
| 34 | 20% (wt/vol) TiCl3 | 37.5 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 35 |
Nitrogen
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Resazurin (0.1% solution)
Dose: 640 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
1M KOH solution
Dose: variable VARIABLE
Role: Mineral source; Properties: Defined component, Solution
NaOH solution
Dose: variable VARIABLE
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ls4d (normalized source unresolved)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.321588Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2809 |
| 2026-01-28T19:48:27.365890Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: 1M KOH solution; Added default concentration for ingredient: N2; Added default concentration for ingredient: NaOH solution; Added default concentration for ingredient: Nitrogen |
| 2026-03-09T08:04:45.376466+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:15.834033Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:05.843845Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009357 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.768188+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LS4D" to "ls4d" for consistent matching |
| 2026-05-16T09:03:39.235987+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:11.436102+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.585637+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.579539+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ae8bfb264b44c86336aed412ad620f2f6cf414112f32324a402eae16954347e7 |