Overview
- External ontology
- TOGO:M2844 — Semisolid LGI medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2844
Composition
flowchart LR
CultureMech_009386["semisolid_lgi_medium"]
ing0_0_2NKOH["0.2NKOH"]
CultureMech_009386 --> ing0_0_2NKOH
ing1_distilled_water["distilled water"]
CultureMech_009386 --> ing1_distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_distilled_water -.-> CHEBI_15377
ing2_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009386 --> ing2_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4___7H2O -.-> CHEBI_31795
ing3_CaCl2___2H2O["CaCl2 · 2H2O"]
CultureMech_009386 --> ing3_CaCl2___2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2___2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009386 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_009386 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Na2MoO4___2H2O["Na2MoO4 · 2H2O"]
CultureMech_009386 --> ing6_Na2MoO4___2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4___2H2O -.-> CHEBI_75213
ing7_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_009386 --> ing7_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_6H2O -.-> CHEBI_86254
ing8_agar["agar"]
CultureMech_009386 --> ing8_agar
CHEBI_2509["CHEBI:2509"]
ing8_agar -.-> CHEBI_2509
ing9_bromothymol_blue["bromothymol blue"]
CultureMech_009386 --> ing9_bromothymol_blue
CHEBI_86155["CHEBI:86155"]
ing9_bromothymol_blue -.-> CHEBI_86155
ing10_crystallized_cane_sugar["crystallized cane sugar"]
CultureMech_009386 --> ing10_crystallized_cane_sugar
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 0.2NKOH | 5 G_PER_L | Role: Mineral source; Properties: Defined component, Solution | |
| 2 |
distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
CaCl2 · 2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Na2MoO4 · 2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.002 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.01 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 | agar | 1.8 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 10 |
bromothymol blue
C27H28Br2O5S 624.391 g/mol
|
0.5 PERCENT_W_V |
CHEBI:86155
bromothymol blue
|
Role: pH indicator |
| 11 | crystallized cane sugar | 100 G_PER_L | Role: Nutrient source |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- semisolid_lgi_medium (CultureMech:009386)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.349360Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2844 |
| 2026-01-28T19:48:22.243904Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:05.990292Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009386 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.922023+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Semisolid LGI medium" to "semisolid_lgi_medium" for consistent matching |
| 2026-06-05T07:41:45.716115+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:32.135398+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:11.446184+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.689100+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 43077db2af4aca8f797dfbcf28159ee9bc410c44da001c4122db5a0b35286805 |