Overview
- External ontology
- TOGO:M2846 — minimal mineral medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2846
Composition
flowchart LR
CultureMech_009388["minimal_mineral_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009388 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4___7H2O["MgSO4 · 7H2O"]
CultureMech_009388 --> ing1_MgSO4___7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4___7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009388 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_009388 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Na2HPO4["Na2HPO4"]
CultureMech_009388 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Na2MoO4___H2O["Na2MoO4 · H2O"]
CultureMech_009388 --> ing5_Na2MoO4___H2O
CHEBI_75215["CHEBI:75215"]
ing5_Na2MoO4___H2O -.-> CHEBI_75215
ing6_H3BO3["H3BO3"]
CultureMech_009388 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009388 --> ing7_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_7H2O -.-> CHEBI_75836
ing8_CuCl2___H2O["CuCl2 · H2O"]
CultureMech_009388 --> ing8_CuCl2___H2O
CHEBI_49553["CHEBI:49553"]
ing8_CuCl2___H2O -.-> CHEBI_49553
ing9_ZnCl2["ZnCl2"]
CultureMech_009388 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnSO4___H2O["MnSO4 · H2O"]
CultureMech_009388 --> ing10_MnSO4___H2O
CHEBI_86364["CHEBI:86364"]
ing10_MnSO4___H2O -.-> CHEBI_86364
ing11_Co_NO3_2___6H2O["Co(NO3)2 · 6H2O"]
CultureMech_009388 --> ing11_Co_NO3_2___6H2O
CHEBI_86214["CHEBI:86214"]
ing11_Co_NO3_2___6H2O -.-> CHEBI_86214
ing12_NiSO4_6H2O["NiSO4·6H2O"]
CultureMech_009388 --> ing12_NiSO4_6H2O
CHEBI_53001["CHEBI:53001"]
ing12_NiSO4_6H2O -.-> CHEBI_53001
ing13_H2SO4["H2SO4"]
CultureMech_009388 --> ing13_H2SO4
CHEBI_26836["CHEBI:26836"]
ing13_H2SO4 -.-> CHEBI_26836
ing14_NH4VO3["NH4VO3"]
CultureMech_009388 --> ing14_NH4VO3
ing15_Ca_NO3_2["Ca(NO3)2"]
CultureMech_009388 --> ing15_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing15_Ca_NO3_2 -.-> CHEBI_64205
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4 · 7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.9 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
5.1 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2MoO4 · H2O
MoO4.2Na 205.916 g/mol
|
0.25 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CuCl2 · H2O
Cl2Cu 134.452 g/mol
|
0.25 G_PER_L |
CHEBI:49553
copper(II) chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.25 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnSO4 · H2O
H2O.Mn.O4S 169.016 g/mol
|
1 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Co(NO3)2 · 6H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.25 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NiSO4·6H2O
Ni.O4S 154.756 g/mol
|
0.1 G_PER_L |
CHEBI:53001
nickel sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H2SO4
H2O4S 98.079 g/mol
|
9.2 G_PER_L |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
| 15 | NH4VO3 | 0.1 G_PER_L | Role: Mineral source | |
| 16 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
25 G_PER_L |
CHEBI:64205
calcium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
trace element solution
Dose: 1 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
solution of Ca(NO3)2
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_mineral_medium (CultureMech:009388)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.351744Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2846 |
| 2026-01-28T19:48:11.058320Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:18.119431+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:45.786022Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:06.002074Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009388 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:24.934380+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "minimal mineral medium" to "minimal_mineral_medium" for consistent matching |
| 2026-05-16T09:04:24.097304+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:34.776904+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:48.550082+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:58:24.774575+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.073181+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.481285+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 630b483a5e9b0fdeadb46f5100722771635d437d0b4e0f3f85fd11f4b9d84264 |