Overview
- External ontology
- TOGO:M2885 — Minimal medium containing nicotin
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2885
Composition
flowchart LR
CultureMech_009420["minimal_medium_containing_nicotin"]
ing0_Distilled_water["Distilled water"]
CultureMech_009420 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4.7H2O"]
CultureMech_009420 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009420 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4_3H2O["K2HPO4.3H2O"]
CultureMech_009420 --> ing3_K2HPO4_3H2O
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4_3H2O -.-> CHEBI_131527
ing4_Nicotine__Fluka_["Nicotine (Fluka)"]
CultureMech_009420 --> ing4_Nicotine__Fluka_
ing5_0_1M_HCl["0.1M HCl"]
CultureMech_009420 --> ing5_0_1M_HCl
ing6_CaCl2_2H2O["CaCl2.2H2O"]
CultureMech_009420 --> ing6_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_2H2O -.-> CHEBI_86158
ing7_Na2MoO4_2H2O["Na2MoO4.2H2O"]
CultureMech_009420 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_FeSO4_7H2O["FeSO4.7H2O"]
CultureMech_009420 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_CuCl2_2H2O["CuCl2.2H2O"]
CultureMech_009420 --> ing9_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing9_CuCl2_2H2O -.-> CHEBI_86318
ing10_Na2WO4_2H2O["Na2WO4.2H2O"]
CultureMech_009420 --> ing10_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing10_Na2WO4_2H2O -.-> CHEBI_63939
ing11_MnSO4_H2O["MnSO4.H2O"]
CultureMech_009420 --> ing11_MnSO4_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_H2O -.-> CHEBI_86364
ing12_ZnSO4["ZnSO4"]
CultureMech_009420 --> ing12_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing12_ZnSO4 -.-> CHEBI_35176
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4.7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
K2HPO4.3H2O
HO4P.2K 174.174 g/mol
|
13.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 | Nicotine (Fluka) | 3 G_PER_L | Role: Nutrient source; Properties: Organic compound, Simple component, Defined component | |
| 6 | 0.1M HCl | 1 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 7 |
CaCl2.2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2MoO4.2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 9 |
FeSO4.7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 10 |
CuCl2.2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
Na2WO4.2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.05 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 12 |
MnSO4.H2O
H2O.Mn.O4S 169.016 g/mol
|
0.008 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 13 |
ZnSO4
O4S.Zn 161.453 g/mol
|
0.1 G_PER_L |
CHEBI:35176
zinc sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Trace elements solution
Dose: 0.5 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- minimal_medium_containing_nicotin (CultureMech:009420)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.379273Z | togo-import | Imported from TOGO Medium | Source: TOGO, ID: M2885 |
| 2026-01-28T19:48:38.780862Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:04.942133+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:06.121101Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009420 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:25.072104+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Minimal medium containing nicotin" to "minimal_medium_containing_nicotin" for consistent matching |
| 2026-05-16T09:04:24.027944+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:34.668041+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.069026+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.341390+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 111a1d548ed0cc0ffe73a4b217fde088831fcdeca1a9e980a9e32beb2e59ea31 |